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Identification methods for irradiated wheat

Abstract

The effect of irradiation on wheat seeds was examined using various kinds of analytical methods for the identification of irradiated seeds. In germination test, the growth of sprouts was markedly inhibited at 500Gy, which was not affected by storage. The decrease in germination percentage was detected at 3300Gy. The results of enzymatic activity change in the germ measured by Vita-Scope germinator showed that the seeds irradiated at 10kGy could be identified. The content of amino acids in ungerminated and germinated seeds were analyzed. Irradiation at 10kGy caused the decrease of lysine content but the change was small which need very careful operation to detect it. The chemiluminescence intensity increased with radiation dose and decreased during storage. The wheat irradiated at 10kGy could be identified even after 3 months storage. In the electron spin resonance (ESR) spectrum analysis, the signal intensity with the g value f 2.0055 of skinned wheat seeds increased with radiation dose. Among these methods, germination test was the most sensitive and effective for identification of irradiated wheat. (author).
Authors:
Shengtao, Zhu; [1]  Kume, Tamikazu; Ishigaki, Isao
  1. Suzhou Medical Coll., JS (China)
Publication Date:
Feb 01, 1992
Product Type:
Technical Report
Report Number:
JAERI-M-92-014
Reference Number:
SCA: 553004; PA: JPN-92:005139; SN: 92000757147
Resource Relation:
Other Information: PBD: Feb 1992
Subject:
60 APPLIED LIFE SCIENCES; WHEAT; BIOLOGICAL RADIATION EFFECTS; SEEDS; GERMINATION; DOSE-RESPONSE RELATIONSHIPS; ELECTRON SPIN RESONANCE; STORAGE; SENSITIVITY; CHEMILUMINESCENCE; SPROUT INHIBITION; LYSINE; GAMMA RADIATION; IRRADIATION; 553004; FOOD PROTECTION AND PRESERVATION
OSTI ID:
10149619
Research Organizations:
Japan Atomic Energy Research Inst., Tokyo (Japan)
Country of Origin:
Japan
Language:
English
Other Identifying Numbers:
Other: ON: DE92526852; TRN: JP9205139
Availability:
OSTI; NTIS; INIS
Submitting Site:
JPN
Size:
31 p.
Announcement Date:
Jul 05, 2005

Citation Formats

Shengtao, Zhu, Kume, Tamikazu, and Ishigaki, Isao. Identification methods for irradiated wheat. Japan: N. p., 1992. Web.
Shengtao, Zhu, Kume, Tamikazu, & Ishigaki, Isao. Identification methods for irradiated wheat. Japan.
Shengtao, Zhu, Kume, Tamikazu, and Ishigaki, Isao. 1992. "Identification methods for irradiated wheat." Japan.
@misc{etde_10149619,
title = {Identification methods for irradiated wheat}
author = {Shengtao, Zhu, Kume, Tamikazu, and Ishigaki, Isao}
abstractNote = {The effect of irradiation on wheat seeds was examined using various kinds of analytical methods for the identification of irradiated seeds. In germination test, the growth of sprouts was markedly inhibited at 500Gy, which was not affected by storage. The decrease in germination percentage was detected at 3300Gy. The results of enzymatic activity change in the germ measured by Vita-Scope germinator showed that the seeds irradiated at 10kGy could be identified. The content of amino acids in ungerminated and germinated seeds were analyzed. Irradiation at 10kGy caused the decrease of lysine content but the change was small which need very careful operation to detect it. The chemiluminescence intensity increased with radiation dose and decreased during storage. The wheat irradiated at 10kGy could be identified even after 3 months storage. In the electron spin resonance (ESR) spectrum analysis, the signal intensity with the g value f 2.0055 of skinned wheat seeds increased with radiation dose. Among these methods, germination test was the most sensitive and effective for identification of irradiated wheat. (author).}
place = {Japan}
year = {1992}
month = {Feb}
}