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Thrombomodulin and von Willebrand factor as markers of radiation-induced endothelial injury

Abstract

Cultured confluent human umbilical vein endothelial cells were irradiated in vitro by {sup 60}Co-gamma ray at doses from 0 to 50 Gy. After irradiation Thrombomodulin in the supernatants of endothelial cell culture medium, on the surface of the cells and within the cells was measured at different times over six days. At twenty-four hours after irradiation, an increase in the release of Thrombomodulin and von Willebrand factor from irradiated endothelial cells and an increase in the number of molecules and the activity of Thrombomodulin on the surface of the cells were observed, which were radiation-dose dependent. The capacity of the cells to produce and release Thrombomodulin was decreased from two to six days after exposure to {sup 60}Co-gamma ray. Our data indicate that radiation can injure endothelial cells and that Thrombomodulin may be as a marker of radiation-induced endothelial cell injury. The relationship between dysfunction of irradiated endothelial cells and the pathological mechanisms of acute radiation sickness are discussed.
Authors:
Quansheng, Zhou; Yimin, Zhao; Peixia, Li; Xia, Bai; Changgeng, Ruan [1] 
  1. Suzhou Medical College (China)
Publication Date:
Feb 01, 1992
Product Type:
Technical Report
Report Number:
CNIC-00585; SMC-0072.
Reference Number:
SCA: 560120; PA: AIX-24:037898; SN: 93000979547
Resource Relation:
Other Information: PBD: Feb 1992
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ENDOTHELIUM; RADIATION INJURIES; CELL CULTURES; CELL MEMBRANES; DOSE-RESPONSE RELATIONSHIPS; GAMMA RADIATION; THROMBOSIS; VEINS; 560120; RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
OSTI ID:
10145174
Research Organizations:
China Nuclear Information Centre, Beijing, BJ (China)
Country of Origin:
China
Language:
English
Other Identifying Numbers:
Other: ON: DE93623414; TRN: CN9300209037898
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
[12] p.
Announcement Date:
Jul 05, 2005

Citation Formats

Quansheng, Zhou, Yimin, Zhao, Peixia, Li, Xia, Bai, and Changgeng, Ruan. Thrombomodulin and von Willebrand factor as markers of radiation-induced endothelial injury. China: N. p., 1992. Web.
Quansheng, Zhou, Yimin, Zhao, Peixia, Li, Xia, Bai, & Changgeng, Ruan. Thrombomodulin and von Willebrand factor as markers of radiation-induced endothelial injury. China.
Quansheng, Zhou, Yimin, Zhao, Peixia, Li, Xia, Bai, and Changgeng, Ruan. 1992. "Thrombomodulin and von Willebrand factor as markers of radiation-induced endothelial injury." China.
@misc{etde_10145174,
title = {Thrombomodulin and von Willebrand factor as markers of radiation-induced endothelial injury}
author = {Quansheng, Zhou, Yimin, Zhao, Peixia, Li, Xia, Bai, and Changgeng, Ruan}
abstractNote = {Cultured confluent human umbilical vein endothelial cells were irradiated in vitro by {sup 60}Co-gamma ray at doses from 0 to 50 Gy. After irradiation Thrombomodulin in the supernatants of endothelial cell culture medium, on the surface of the cells and within the cells was measured at different times over six days. At twenty-four hours after irradiation, an increase in the release of Thrombomodulin and von Willebrand factor from irradiated endothelial cells and an increase in the number of molecules and the activity of Thrombomodulin on the surface of the cells were observed, which were radiation-dose dependent. The capacity of the cells to produce and release Thrombomodulin was decreased from two to six days after exposure to {sup 60}Co-gamma ray. Our data indicate that radiation can injure endothelial cells and that Thrombomodulin may be as a marker of radiation-induced endothelial cell injury. The relationship between dysfunction of irradiated endothelial cells and the pathological mechanisms of acute radiation sickness are discussed.}
place = {China}
year = {1992}
month = {Feb}
}