Abstract
A bioassay method was developed for directly measuring organically bound tritium (OBT) in urine and feces. Samples first undergo low-temperature distillation and vacuum separation to isolate tritiated water (HTO) and exchangeable tritium. This is followed by converting the non-exchangeable tritium (i.e., OBT) into HTO through oxygen combustion. The method was investigated to: optimise the sample preparation procedures; establish OBT recovery (64% {+-} 7% for urine and 71% {+-} 8% for feces); and, determine the detection limit for OBT in urine (0.3 Bq {center_dot} g{sup -1}) and feces (5 Bq {center_dot} g{sup -1}). The method was evaluated for error sources that are associated with the exchange between HTO and OBT. It is concluded that this bioassay method can reliably measure OBT in urine and feces within the range of {+-} 10%.
Citation Formats
Trivedi, A, Duong, T, Leon, J W, and Linauskas, S H.
Measurement of organically bound tritium in urine and feces.
Canada: N. p.,
1993.
Web.
Trivedi, A, Duong, T, Leon, J W, & Linauskas, S H.
Measurement of organically bound tritium in urine and feces.
Canada.
Trivedi, A, Duong, T, Leon, J W, and Linauskas, S H.
1993.
"Measurement of organically bound tritium in urine and feces."
Canada.
@misc{etde_10138625,
title = {Measurement of organically bound tritium in urine and feces}
author = {Trivedi, A, Duong, T, Leon, J W, and Linauskas, S H}
abstractNote = {A bioassay method was developed for directly measuring organically bound tritium (OBT) in urine and feces. Samples first undergo low-temperature distillation and vacuum separation to isolate tritiated water (HTO) and exchangeable tritium. This is followed by converting the non-exchangeable tritium (i.e., OBT) into HTO through oxygen combustion. The method was investigated to: optimise the sample preparation procedures; establish OBT recovery (64% {+-} 7% for urine and 71% {+-} 8% for feces); and, determine the detection limit for OBT in urine (0.3 Bq {center_dot} g{sup -1}) and feces (5 Bq {center_dot} g{sup -1}). The method was evaluated for error sources that are associated with the exchange between HTO and OBT. It is concluded that this bioassay method can reliably measure OBT in urine and feces within the range of {+-} 10%.}
place = {Canada}
year = {1993}
month = {Nov}
}
title = {Measurement of organically bound tritium in urine and feces}
author = {Trivedi, A, Duong, T, Leon, J W, and Linauskas, S H}
abstractNote = {A bioassay method was developed for directly measuring organically bound tritium (OBT) in urine and feces. Samples first undergo low-temperature distillation and vacuum separation to isolate tritiated water (HTO) and exchangeable tritium. This is followed by converting the non-exchangeable tritium (i.e., OBT) into HTO through oxygen combustion. The method was investigated to: optimise the sample preparation procedures; establish OBT recovery (64% {+-} 7% for urine and 71% {+-} 8% for feces); and, determine the detection limit for OBT in urine (0.3 Bq {center_dot} g{sup -1}) and feces (5 Bq {center_dot} g{sup -1}). The method was evaluated for error sources that are associated with the exchange between HTO and OBT. It is concluded that this bioassay method can reliably measure OBT in urine and feces within the range of {+-} 10%.}
place = {Canada}
year = {1993}
month = {Nov}
}