Abstract
In the present work different concentrations of methyl-{sup 3} H-thymidine was added to the culture medium micro drops containing the mouse zygotes at pro nuclear stage and the embryos were cultured in vitro at 37{sup 0} C in a humidified atmosphere with 5% of CO{sub 2} for four days up to the expanded blastocyst stage, the established end point to calculate the L D{sub 50} lethal dose. The blastocyst formation rate decreased with increasing concentration of tritium in medium and a value of 2.4 X 10{sup 3} Bq/ml for L D{sub 50} was obtained. The {sup 3} H-Td R incorporation by the embryo during the preimplantation period was low at the beginning and increased quickly during the morula and the blastocyst development. (author).
Kikuchi, O K;
[1]
Ohyama, H;
Yamada, T
[2]
- Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)
- National Institute of Radiological Sciences, Chiba (Japan)
Citation Formats
Kikuchi, O K, Ohyama, H, and Yamada, T.
Radiotoxicity and incorporation of methyl-tritiated-thymidine on preimplantation mouse embryo. In vitro fertilization and culture; Radiotoxicidade e incorporacao de timidina-metil-tritiada em embrioes de camundongo na fase de pre-implantacao. Cultura e fertilizacao in vitro.
Brazil: N. p.,
1993.
Web.
Kikuchi, O K, Ohyama, H, & Yamada, T.
Radiotoxicity and incorporation of methyl-tritiated-thymidine on preimplantation mouse embryo. In vitro fertilization and culture; Radiotoxicidade e incorporacao de timidina-metil-tritiada em embrioes de camundongo na fase de pre-implantacao. Cultura e fertilizacao in vitro.
Brazil.
Kikuchi, O K, Ohyama, H, and Yamada, T.
1993.
"Radiotoxicity and incorporation of methyl-tritiated-thymidine on preimplantation mouse embryo. In vitro fertilization and culture; Radiotoxicidade e incorporacao de timidina-metil-tritiada em embrioes de camundongo na fase de pre-implantacao. Cultura e fertilizacao in vitro."
Brazil.
@misc{etde_10130511,
title = {Radiotoxicity and incorporation of methyl-tritiated-thymidine on preimplantation mouse embryo. In vitro fertilization and culture; Radiotoxicidade e incorporacao de timidina-metil-tritiada em embrioes de camundongo na fase de pre-implantacao. Cultura e fertilizacao in vitro}
author = {Kikuchi, O K, Ohyama, H, and Yamada, T}
abstractNote = {In the present work different concentrations of methyl-{sup 3} H-thymidine was added to the culture medium micro drops containing the mouse zygotes at pro nuclear stage and the embryos were cultured in vitro at 37{sup 0} C in a humidified atmosphere with 5% of CO{sub 2} for four days up to the expanded blastocyst stage, the established end point to calculate the L D{sub 50} lethal dose. The blastocyst formation rate decreased with increasing concentration of tritium in medium and a value of 2.4 X 10{sup 3} Bq/ml for L D{sub 50} was obtained. The {sup 3} H-Td R incorporation by the embryo during the preimplantation period was low at the beginning and increased quickly during the morula and the blastocyst development. (author).}
place = {Brazil}
year = {1993}
month = {Apr}
}
title = {Radiotoxicity and incorporation of methyl-tritiated-thymidine on preimplantation mouse embryo. In vitro fertilization and culture; Radiotoxicidade e incorporacao de timidina-metil-tritiada em embrioes de camundongo na fase de pre-implantacao. Cultura e fertilizacao in vitro}
author = {Kikuchi, O K, Ohyama, H, and Yamada, T}
abstractNote = {In the present work different concentrations of methyl-{sup 3} H-thymidine was added to the culture medium micro drops containing the mouse zygotes at pro nuclear stage and the embryos were cultured in vitro at 37{sup 0} C in a humidified atmosphere with 5% of CO{sub 2} for four days up to the expanded blastocyst stage, the established end point to calculate the L D{sub 50} lethal dose. The blastocyst formation rate decreased with increasing concentration of tritium in medium and a value of 2.4 X 10{sup 3} Bq/ml for L D{sub 50} was obtained. The {sup 3} H-Td R incorporation by the embryo during the preimplantation period was low at the beginning and increased quickly during the morula and the blastocyst development. (author).}
place = {Brazil}
year = {1993}
month = {Apr}
}