Abstract
DANTE-Z is a simple and efficient way for NMR spectral selection. We present here different applications of DANTE-Z in high-resolution NMR of peptides and proteins. We have been using proton selective excitation by DANTE-Z to perform 1D-correlated (homo- or heteronuclear) experiments corresponding to one line of either 2D or 3D experiments. Following the same scheme, we could also edit planes of 3D experiments by concatenating 1D-correlated experiments with conventional 2D experiments. In the heteronuclear case (i.e. {sup 1}H, {sup 31}P), we could also edit planes of a 4D experiment by the simultaneous selection of {sup 1}H and the X nucleus. Owing to the favourable excitation profile of DANTE-Z, we used it successfully for topological excitations (spectral width from 150 Hz up to 1500 Hz) in `semi-soft`-2D experiments and `soft`-2D experiment. These applications are illustrated by the results obtained at 600 MHz on a protein and a phosphonamide peptide.
Roumestand, C;
Toma, F;
[1]
Boudot, D
[2]
- CEA Centre d`Etudes de Saclay, 91 - Gif-sur-Yvette (France). Direction des Sciences du Vivant
- Nancy-1 Univ., 54 (France)
Citation Formats
Roumestand, C, Toma, F, and Boudot, D.
Dante-Z sequence as selective impulsion in high field mono and multidimensional NMR. Application to the study of proteins, peptides and their interactions; Utilisation de la sequence Dante-Z comme impulsion selective en RMN haute resolution mono et multidimensionnelle. Application a l`etude des proteines, des peptides et de leurs interactions.
France: N. p.,
1992.
Web.
Roumestand, C, Toma, F, & Boudot, D.
Dante-Z sequence as selective impulsion in high field mono and multidimensional NMR. Application to the study of proteins, peptides and their interactions; Utilisation de la sequence Dante-Z comme impulsion selective en RMN haute resolution mono et multidimensionnelle. Application a l`etude des proteines, des peptides et de leurs interactions.
France.
Roumestand, C, Toma, F, and Boudot, D.
1992.
"Dante-Z sequence as selective impulsion in high field mono and multidimensional NMR. Application to the study of proteins, peptides and their interactions; Utilisation de la sequence Dante-Z comme impulsion selective en RMN haute resolution mono et multidimensionnelle. Application a l`etude des proteines, des peptides et de leurs interactions."
France.
@misc{etde_10129401,
title = {Dante-Z sequence as selective impulsion in high field mono and multidimensional NMR. Application to the study of proteins, peptides and their interactions; Utilisation de la sequence Dante-Z comme impulsion selective en RMN haute resolution mono et multidimensionnelle. Application a l`etude des proteines, des peptides et de leurs interactions}
author = {Roumestand, C, Toma, F, and Boudot, D}
abstractNote = {DANTE-Z is a simple and efficient way for NMR spectral selection. We present here different applications of DANTE-Z in high-resolution NMR of peptides and proteins. We have been using proton selective excitation by DANTE-Z to perform 1D-correlated (homo- or heteronuclear) experiments corresponding to one line of either 2D or 3D experiments. Following the same scheme, we could also edit planes of 3D experiments by concatenating 1D-correlated experiments with conventional 2D experiments. In the heteronuclear case (i.e. {sup 1}H, {sup 31}P), we could also edit planes of a 4D experiment by the simultaneous selection of {sup 1}H and the X nucleus. Owing to the favourable excitation profile of DANTE-Z, we used it successfully for topological excitations (spectral width from 150 Hz up to 1500 Hz) in `semi-soft`-2D experiments and `soft`-2D experiment. These applications are illustrated by the results obtained at 600 MHz on a protein and a phosphonamide peptide.}
place = {France}
year = {1992}
month = {Dec}
}
title = {Dante-Z sequence as selective impulsion in high field mono and multidimensional NMR. Application to the study of proteins, peptides and their interactions; Utilisation de la sequence Dante-Z comme impulsion selective en RMN haute resolution mono et multidimensionnelle. Application a l`etude des proteines, des peptides et de leurs interactions}
author = {Roumestand, C, Toma, F, and Boudot, D}
abstractNote = {DANTE-Z is a simple and efficient way for NMR spectral selection. We present here different applications of DANTE-Z in high-resolution NMR of peptides and proteins. We have been using proton selective excitation by DANTE-Z to perform 1D-correlated (homo- or heteronuclear) experiments corresponding to one line of either 2D or 3D experiments. Following the same scheme, we could also edit planes of 3D experiments by concatenating 1D-correlated experiments with conventional 2D experiments. In the heteronuclear case (i.e. {sup 1}H, {sup 31}P), we could also edit planes of a 4D experiment by the simultaneous selection of {sup 1}H and the X nucleus. Owing to the favourable excitation profile of DANTE-Z, we used it successfully for topological excitations (spectral width from 150 Hz up to 1500 Hz) in `semi-soft`-2D experiments and `soft`-2D experiment. These applications are illustrated by the results obtained at 600 MHz on a protein and a phosphonamide peptide.}
place = {France}
year = {1992}
month = {Dec}
}