Abstract
In order to check whether the increase of celular viability observed in cultures of Escherichia coli and Salmonella typhimurium, irradiated with ultraviolet and incubated in non nutrient medium, would be due to cell multiplication, and/or repair we applied a Statistical Fluctuation Test, based on Poisson Distribution. Utilizing macromolecules in strains that show true liquid holding recovery (LHR) or cell multiplication. Our results show that cell multiplication and not repair occurs in non nutrient medium for E.coli AB2470 (rrecB21), E.coli JG112 (polA1) an in the {Delta}uvrB mutant of S.typhimurium. In E.coli JG112, the multiplication rate is higher when thymine is added to the non nutrient medium, due to the auxotrophism of this strain. In E.coli lexB30 mutant, we observed repair in non nutrient medium (LHR) and cell multiplication, while in E.coli lexA1 mutant only LHR was observed. Studies of macromolecules degradation indicate that the final products are, probably reutilized by the cells, creating possibility of multiplication and/or repair. (author).
Citation Formats
Bezerra, R J.A.
Restoration in non nutrient medium: relative importance of some separation genetic markers; Restauracao em meio nao nutriente: importancia relativa de alguns marcadores geneticos de reparacao.
Brazil: N. p.,
1980.
Web.
Bezerra, R J.A.
Restoration in non nutrient medium: relative importance of some separation genetic markers; Restauracao em meio nao nutriente: importancia relativa de alguns marcadores geneticos de reparacao.
Brazil.
Bezerra, R J.A.
1980.
"Restoration in non nutrient medium: relative importance of some separation genetic markers; Restauracao em meio nao nutriente: importancia relativa de alguns marcadores geneticos de reparacao."
Brazil.
@misc{etde_10127972,
title = {Restoration in non nutrient medium: relative importance of some separation genetic markers; Restauracao em meio nao nutriente: importancia relativa de alguns marcadores geneticos de reparacao}
author = {Bezerra, R J.A.}
abstractNote = {In order to check whether the increase of celular viability observed in cultures of Escherichia coli and Salmonella typhimurium, irradiated with ultraviolet and incubated in non nutrient medium, would be due to cell multiplication, and/or repair we applied a Statistical Fluctuation Test, based on Poisson Distribution. Utilizing macromolecules in strains that show true liquid holding recovery (LHR) or cell multiplication. Our results show that cell multiplication and not repair occurs in non nutrient medium for E.coli AB2470 (rrecB21), E.coli JG112 (polA1) an in the {Delta}uvrB mutant of S.typhimurium. In E.coli JG112, the multiplication rate is higher when thymine is added to the non nutrient medium, due to the auxotrophism of this strain. In E.coli lexB30 mutant, we observed repair in non nutrient medium (LHR) and cell multiplication, while in E.coli lexA1 mutant only LHR was observed. Studies of macromolecules degradation indicate that the final products are, probably reutilized by the cells, creating possibility of multiplication and/or repair. (author).}
place = {Brazil}
year = {1980}
month = {Dec}
}
title = {Restoration in non nutrient medium: relative importance of some separation genetic markers; Restauracao em meio nao nutriente: importancia relativa de alguns marcadores geneticos de reparacao}
author = {Bezerra, R J.A.}
abstractNote = {In order to check whether the increase of celular viability observed in cultures of Escherichia coli and Salmonella typhimurium, irradiated with ultraviolet and incubated in non nutrient medium, would be due to cell multiplication, and/or repair we applied a Statistical Fluctuation Test, based on Poisson Distribution. Utilizing macromolecules in strains that show true liquid holding recovery (LHR) or cell multiplication. Our results show that cell multiplication and not repair occurs in non nutrient medium for E.coli AB2470 (rrecB21), E.coli JG112 (polA1) an in the {Delta}uvrB mutant of S.typhimurium. In E.coli JG112, the multiplication rate is higher when thymine is added to the non nutrient medium, due to the auxotrophism of this strain. In E.coli lexB30 mutant, we observed repair in non nutrient medium (LHR) and cell multiplication, while in E.coli lexA1 mutant only LHR was observed. Studies of macromolecules degradation indicate that the final products are, probably reutilized by the cells, creating possibility of multiplication and/or repair. (author).}
place = {Brazil}
year = {1980}
month = {Dec}
}