Abstract
Seeds of the rice variety Krispo-38 were irradiated with 0, 100, 200, 300 and 400 Gray (Gy) and sown in the greenhouse. From each treatment, others containing uninucleate pollen grains were collected and cultured on a N6 agar medium with 5% sucrose concentration and supplemented with mg/l naphthalene acetic acid (NAA), 1 mg/l kinetin and 1 mg/l 2,4 dichlorophenoxyacetic acid (2,4-D). The pollen grains were induced to develop callus. The percentage of others that produced calli varied from 0.8 for the 400-Gy treatment to 3.3% for the control. The calli were transferred to N6 medium with 3% sucrose concentration, supplemented with 0.5 mg/l NAA and 1 mg/l kinetin. The percentage of calli that produced green plants varied from 1.9 for the 300-Gy treatment to 10.5 for the 200 Gy treatment. Plants developed in 2-5 weeks after callus transplant. A total of 101 green plants was obtained.
Montepeque, R;
Molina, L G;
Lopez, J J;
[1]
Pazos, W;
Ramirez, J
[2]
- Seccion Agropecuaria, Direccion General de Energia Nuclear (Guatemala)
- Instituto de Ciencia y Tecnologia Agricola (Guatemala)
Citation Formats
Montepeque, R, Molina, L G, Lopez, J J, Pazos, W, and Ramirez, J.
Radiation effect in another culture rice (Oryza Sativa L.) variety Krispo-38; Efecto de la radiacion en el cultivo de anteras de arroz (Oriza Sativa) variedad Krispo-38.
Guatemala: N. p.,
1993.
Web.
Montepeque, R, Molina, L G, Lopez, J J, Pazos, W, & Ramirez, J.
Radiation effect in another culture rice (Oryza Sativa L.) variety Krispo-38; Efecto de la radiacion en el cultivo de anteras de arroz (Oriza Sativa) variedad Krispo-38.
Guatemala.
Montepeque, R, Molina, L G, Lopez, J J, Pazos, W, and Ramirez, J.
1993.
"Radiation effect in another culture rice (Oryza Sativa L.) variety Krispo-38; Efecto de la radiacion en el cultivo de anteras de arroz (Oriza Sativa) variedad Krispo-38."
Guatemala.
@misc{etde_10122647,
title = {Radiation effect in another culture rice (Oryza Sativa L.) variety Krispo-38; Efecto de la radiacion en el cultivo de anteras de arroz (Oriza Sativa) variedad Krispo-38}
author = {Montepeque, R, Molina, L G, Lopez, J J, Pazos, W, and Ramirez, J}
abstractNote = {Seeds of the rice variety Krispo-38 were irradiated with 0, 100, 200, 300 and 400 Gray (Gy) and sown in the greenhouse. From each treatment, others containing uninucleate pollen grains were collected and cultured on a N6 agar medium with 5% sucrose concentration and supplemented with mg/l naphthalene acetic acid (NAA), 1 mg/l kinetin and 1 mg/l 2,4 dichlorophenoxyacetic acid (2,4-D). The pollen grains were induced to develop callus. The percentage of others that produced calli varied from 0.8 for the 400-Gy treatment to 3.3% for the control. The calli were transferred to N6 medium with 3% sucrose concentration, supplemented with 0.5 mg/l NAA and 1 mg/l kinetin. The percentage of calli that produced green plants varied from 1.9 for the 300-Gy treatment to 10.5 for the 200 Gy treatment. Plants developed in 2-5 weeks after callus transplant. A total of 101 green plants was obtained.}
place = {Guatemala}
year = {1993}
month = {Dec}
}
title = {Radiation effect in another culture rice (Oryza Sativa L.) variety Krispo-38; Efecto de la radiacion en el cultivo de anteras de arroz (Oriza Sativa) variedad Krispo-38}
author = {Montepeque, R, Molina, L G, Lopez, J J, Pazos, W, and Ramirez, J}
abstractNote = {Seeds of the rice variety Krispo-38 were irradiated with 0, 100, 200, 300 and 400 Gray (Gy) and sown in the greenhouse. From each treatment, others containing uninucleate pollen grains were collected and cultured on a N6 agar medium with 5% sucrose concentration and supplemented with mg/l naphthalene acetic acid (NAA), 1 mg/l kinetin and 1 mg/l 2,4 dichlorophenoxyacetic acid (2,4-D). The pollen grains were induced to develop callus. The percentage of others that produced calli varied from 0.8 for the 400-Gy treatment to 3.3% for the control. The calli were transferred to N6 medium with 3% sucrose concentration, supplemented with 0.5 mg/l NAA and 1 mg/l kinetin. The percentage of calli that produced green plants varied from 1.9 for the 300-Gy treatment to 10.5 for the 200 Gy treatment. Plants developed in 2-5 weeks after callus transplant. A total of 101 green plants was obtained.}
place = {Guatemala}
year = {1993}
month = {Dec}
}