Abstract
In the course of this study investigations were carried out into the reactions of hydroxyl radicals and hydrogen atoms with cyclic dipeptides as well as the subsequent reactions of peptide radicals and their peroxyl radicals in aqueous solution. The radiolysis products formed in the absence and presence of oxygen or transient metal complexes were characterized and determined on a quantitative basis. The linking of information from product analyses to the kinetic data for transient species obtained by time-resolving UV/VIS and conductivity measurements (pulse radiolysis) as well as computer-assisted simulations of individual events during the reaction permitted an evaluation of the mechanisms underlying the various processes and an identification of interim products with short life-times, which did or did not belong to the group of radicals. Through the characterization of key reactions of radicals and peroxyl radicals of this substance class a major advance has been made towards a better understanding of the role of radicals in the peptide compound and the mechanisms involved in indirect radiation effects on long-chain peptides and proteins. (orig.). [Deutsch] Im Rahmen dieser Arbeit wurden die Reaktionen von Hydroxyl-Radikalen und Wasserstoff-Atomen mit cyclischen Dipeptiden und die sich daran anschliessenden Reaktionen der Peptiradikale und ihrer Peroxylradikale in
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Citation Formats
Mieden, O J.
Hydroxyl-radical-induced oxidation of cyclic dipeptides: Reactions of free peptide radicals and their peroxyl radicals. Analysis of end products and fast kinetic processes of transient species; Hydroxylradikal-induzierte Oxidation cyclischer Dipeptide: Reaktionen der freien Peptidradikale und ihrer Peroxylradikale. Analyse der Endprodukte und schnelle Kinetik der Transienten.
Germany: N. p.,
1989.
Web.
Mieden, O J.
Hydroxyl-radical-induced oxidation of cyclic dipeptides: Reactions of free peptide radicals and their peroxyl radicals. Analysis of end products and fast kinetic processes of transient species; Hydroxylradikal-induzierte Oxidation cyclischer Dipeptide: Reaktionen der freien Peptidradikale und ihrer Peroxylradikale. Analyse der Endprodukte und schnelle Kinetik der Transienten.
Germany.
Mieden, O J.
1989.
"Hydroxyl-radical-induced oxidation of cyclic dipeptides: Reactions of free peptide radicals and their peroxyl radicals. Analysis of end products and fast kinetic processes of transient species; Hydroxylradikal-induzierte Oxidation cyclischer Dipeptide: Reaktionen der freien Peptidradikale und ihrer Peroxylradikale. Analyse der Endprodukte und schnelle Kinetik der Transienten."
Germany.
@misc{etde_10121283,
title = {Hydroxyl-radical-induced oxidation of cyclic dipeptides: Reactions of free peptide radicals and their peroxyl radicals. Analysis of end products and fast kinetic processes of transient species; Hydroxylradikal-induzierte Oxidation cyclischer Dipeptide: Reaktionen der freien Peptidradikale und ihrer Peroxylradikale. Analyse der Endprodukte und schnelle Kinetik der Transienten}
author = {Mieden, O J}
abstractNote = {In the course of this study investigations were carried out into the reactions of hydroxyl radicals and hydrogen atoms with cyclic dipeptides as well as the subsequent reactions of peptide radicals and their peroxyl radicals in aqueous solution. The radiolysis products formed in the absence and presence of oxygen or transient metal complexes were characterized and determined on a quantitative basis. The linking of information from product analyses to the kinetic data for transient species obtained by time-resolving UV/VIS and conductivity measurements (pulse radiolysis) as well as computer-assisted simulations of individual events during the reaction permitted an evaluation of the mechanisms underlying the various processes and an identification of interim products with short life-times, which did or did not belong to the group of radicals. Through the characterization of key reactions of radicals and peroxyl radicals of this substance class a major advance has been made towards a better understanding of the role of radicals in the peptide compound and the mechanisms involved in indirect radiation effects on long-chain peptides and proteins. (orig.). [Deutsch] Im Rahmen dieser Arbeit wurden die Reaktionen von Hydroxyl-Radikalen und Wasserstoff-Atomen mit cyclischen Dipeptiden und die sich daran anschliessenden Reaktionen der Peptiradikale und ihrer Peroxylradikale in waessriger Loesung untersucht. Die Radiolyseprodukte in An- und Abwesenheit von Sauerstoff oder Uebergangsmetallkomplexen wurden charakterisiert und quantitativ bestimmt. Die Kombination der einander ergaenzenden Informationen aus der Produktanalyse und der kinetischen Verfolgung der Transienten durch zeitaufgeloeste UV/VIS- und Leitfaehigkeitsmessungen (Pulsradiolyse) und die rechnergestuetzte Simulation einzelner Teile des Reaktionsverlaufs ermoeglichen eine mechanistische Deutung der verschiedenen Prozesse und die Identifizierung der kurzlebigen radikalischen und nicht-radikalischen Zwischenprodukte. Durch die Aufklaerung der Schluesselreaktionen der Radikale und Peroxylradikale dieser Substanzklasse ist ein Zugang zu einem besseren Verstaendnis der Radikalreaktionen des Peptidgeruestes und der Mechanismen der indirekten Strahlenwirkung auf laengerkettige Peptide und Proteine eroeffnet. (orig.).}
issue = {no. 51}
place = {Germany}
year = {1989}
month = {Dec}
}
title = {Hydroxyl-radical-induced oxidation of cyclic dipeptides: Reactions of free peptide radicals and their peroxyl radicals. Analysis of end products and fast kinetic processes of transient species; Hydroxylradikal-induzierte Oxidation cyclischer Dipeptide: Reaktionen der freien Peptidradikale und ihrer Peroxylradikale. Analyse der Endprodukte und schnelle Kinetik der Transienten}
author = {Mieden, O J}
abstractNote = {In the course of this study investigations were carried out into the reactions of hydroxyl radicals and hydrogen atoms with cyclic dipeptides as well as the subsequent reactions of peptide radicals and their peroxyl radicals in aqueous solution. The radiolysis products formed in the absence and presence of oxygen or transient metal complexes were characterized and determined on a quantitative basis. The linking of information from product analyses to the kinetic data for transient species obtained by time-resolving UV/VIS and conductivity measurements (pulse radiolysis) as well as computer-assisted simulations of individual events during the reaction permitted an evaluation of the mechanisms underlying the various processes and an identification of interim products with short life-times, which did or did not belong to the group of radicals. Through the characterization of key reactions of radicals and peroxyl radicals of this substance class a major advance has been made towards a better understanding of the role of radicals in the peptide compound and the mechanisms involved in indirect radiation effects on long-chain peptides and proteins. (orig.). [Deutsch] Im Rahmen dieser Arbeit wurden die Reaktionen von Hydroxyl-Radikalen und Wasserstoff-Atomen mit cyclischen Dipeptiden und die sich daran anschliessenden Reaktionen der Peptiradikale und ihrer Peroxylradikale in waessriger Loesung untersucht. Die Radiolyseprodukte in An- und Abwesenheit von Sauerstoff oder Uebergangsmetallkomplexen wurden charakterisiert und quantitativ bestimmt. Die Kombination der einander ergaenzenden Informationen aus der Produktanalyse und der kinetischen Verfolgung der Transienten durch zeitaufgeloeste UV/VIS- und Leitfaehigkeitsmessungen (Pulsradiolyse) und die rechnergestuetzte Simulation einzelner Teile des Reaktionsverlaufs ermoeglichen eine mechanistische Deutung der verschiedenen Prozesse und die Identifizierung der kurzlebigen radikalischen und nicht-radikalischen Zwischenprodukte. Durch die Aufklaerung der Schluesselreaktionen der Radikale und Peroxylradikale dieser Substanzklasse ist ein Zugang zu einem besseren Verstaendnis der Radikalreaktionen des Peptidgeruestes und der Mechanismen der indirekten Strahlenwirkung auf laengerkettige Peptide und Proteine eroeffnet. (orig.).}
issue = {no. 51}
place = {Germany}
year = {1989}
month = {Dec}
}