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Preparation of the separating agent of solid phase microparticle for radioimmunoassay

Technical Report:

Abstract

An advanced separating technique is being used for radioimmunoassay (RIA). It is simple, rapid and stable. The separating agent is prepared by utilizing polymerization of monomer acrylamide and acrylic acid for producing carrier microparticle with carboxy group. Then using 1-ethyl-3-(3-dimethelaminopropyl) carbodimide as coupling agent, the carboxy group is covalently coupled to the amino-group of antibody to form solid antibody microparticle. The solid antibody microparticles have been prepared for T{sub 3}, T{sub 4} and second antibody. The suspensibility and stability towards temperature as well as the tightness of the precipitate in RIA were evaluated, and the nonspecific binding is lower than 2{approx}3%, the within-assay cv < 5% and between-assay cv < 15%.
Authors:
Qing, Zhu; Zhen, Guo; Youfeng, He [1] 
  1. China Inst. of Atomic Energy, Beijing (China)
Publication Date:
Jan 01, 1992
Product Type:
Technical Report
Report Number:
CNIC-00568; IAE-0098.
Reference Number:
SCA: 550601; 400201; PA: AIX-24:006524; SN: 93000931491
Resource Relation:
Other Information: PBD: Jan 1992
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; ACRYLAMIDE; COPOLYMERIZATION; ACRYLIC ACID; MICROSPHERES; CHEMICAL PREPARATION; RADIOIMMUNOASSAY; ANTIBODIES; ORGANIC NITROGEN COMPOUNDS; PERFORMANCE; 550601; 400201; UNSEALED RADIONUCLIDES IN DIAGNOSTICS; CHEMICAL AND PHYSICOCHEMICAL PROPERTIES
OSTI ID:
10118896
Research Organizations:
China Nuclear Information Centre, Beijing, BJ (China)
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Other: ON: DE93611955; ISBN 7-5022-0634-5; TRN: CN9201659006524
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
[9] p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Qing, Zhu, Zhen, Guo, and Youfeng, He. Preparation of the separating agent of solid phase microparticle for radioimmunoassay. China: N. p., 1992. Web.
Qing, Zhu, Zhen, Guo, & Youfeng, He. Preparation of the separating agent of solid phase microparticle for radioimmunoassay. China.
Qing, Zhu, Zhen, Guo, and Youfeng, He. 1992. "Preparation of the separating agent of solid phase microparticle for radioimmunoassay." China.
@misc{etde_10118896,
title = {Preparation of the separating agent of solid phase microparticle for radioimmunoassay}
author = {Qing, Zhu, Zhen, Guo, and Youfeng, He}
abstractNote = {An advanced separating technique is being used for radioimmunoassay (RIA). It is simple, rapid and stable. The separating agent is prepared by utilizing polymerization of monomer acrylamide and acrylic acid for producing carrier microparticle with carboxy group. Then using 1-ethyl-3-(3-dimethelaminopropyl) carbodimide as coupling agent, the carboxy group is covalently coupled to the amino-group of antibody to form solid antibody microparticle. The solid antibody microparticles have been prepared for T{sub 3}, T{sub 4} and second antibody. The suspensibility and stability towards temperature as well as the tightness of the precipitate in RIA were evaluated, and the nonspecific binding is lower than 2{approx}3%, the within-assay cv < 5% and between-assay cv < 15%.}
place = {China}
year = {1992}
month = {Jan}
}