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Lymphocyte colony forming units and its application to the study of radiosensitivity

Technical Report:

Abstract

Kinetics and radiosensitivity of human lymphocytes were studied by the techniques of monolayer agar culture and liquid culture in vitro. In the experiments of lymphocyte kinetics, PHA was designated as a motogen for T lymphocyte. LPS, MEBC and BSA were chosen as mitogens for B lymphocyte. The data from thses experiments showed that under the alone or combination stimulation of LPS, MRBC and BSA, B lymphocytes developed to form colonies in agar culture (0.3%) with the same manner. The stimulation of LPS to B lymphocytes was most significant. By the day 6 after seeding, the numbers of colonies in agar culture were maximal. Whereas the numbers decreased significantly by the day 8. The number of T lymphocyte colonies increased with culture time within 12 days. The peak of {sup 3}H-TdR incorporation into T lymphocytes in liquid culture occured at 5th day after seeding. The data above-mentioned demonstrated that the kinetics of lymphocytes cultured in two kinds of environments were different. The studies of the radiosensitivity of T lymphocytes showed that the decreasing in the number of colonies and rate of {sup 3}H-TdR incorporation varied in different dose ranges. In the range of 0{approx}1.0 Gy, r = -0.96, D{sub 0} value  More>>
Authors:
Xiangrui, Ma; Tao, Wang; Hongyun, Wang [1] 
  1. Suzhou Medical Coll. (China)
Publication Date:
Jul 01, 1991
Product Type:
Technical Report
Report Number:
CNIC-00531; SMC-0065.
Reference Number:
SCA: 560120; PA: AIX-24:005758; SN: 93000930893
Resource Relation:
Other Information: PBD: Jul 1991
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; CFU; RADIOSENSITIVITY; LABELLED COMPOUNDS; THYMIDINE; TRITIUM; 560120; RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
OSTI ID:
10118799
Research Organizations:
China Nuclear Information Centre, Beijing, BJ (China)
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Other: ON: DE93611420; ISBN 7-5022-0545-4; TRN: CN9201663005758
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
[9] p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Xiangrui, Ma, Tao, Wang, and Hongyun, Wang. Lymphocyte colony forming units and its application to the study of radiosensitivity. China: N. p., 1991. Web.
Xiangrui, Ma, Tao, Wang, & Hongyun, Wang. Lymphocyte colony forming units and its application to the study of radiosensitivity. China.
Xiangrui, Ma, Tao, Wang, and Hongyun, Wang. 1991. "Lymphocyte colony forming units and its application to the study of radiosensitivity." China.
@misc{etde_10118799,
title = {Lymphocyte colony forming units and its application to the study of radiosensitivity}
author = {Xiangrui, Ma, Tao, Wang, and Hongyun, Wang}
abstractNote = {Kinetics and radiosensitivity of human lymphocytes were studied by the techniques of monolayer agar culture and liquid culture in vitro. In the experiments of lymphocyte kinetics, PHA was designated as a motogen for T lymphocyte. LPS, MEBC and BSA were chosen as mitogens for B lymphocyte. The data from thses experiments showed that under the alone or combination stimulation of LPS, MRBC and BSA, B lymphocytes developed to form colonies in agar culture (0.3%) with the same manner. The stimulation of LPS to B lymphocytes was most significant. By the day 6 after seeding, the numbers of colonies in agar culture were maximal. Whereas the numbers decreased significantly by the day 8. The number of T lymphocyte colonies increased with culture time within 12 days. The peak of {sup 3}H-TdR incorporation into T lymphocytes in liquid culture occured at 5th day after seeding. The data above-mentioned demonstrated that the kinetics of lymphocytes cultured in two kinds of environments were different. The studies of the radiosensitivity of T lymphocytes showed that the decreasing in the number of colonies and rate of {sup 3}H-TdR incorporation varied in different dose ranges. In the range of 0{approx}1.0 Gy, r = -0.96, D{sub 0} value was 1.71 Gy for TL-CFC in agar culture, r = -.96, D{sub 0} value was 4.34 Gy for the proliferation T lymphocytes in liquid culture. In the range of 1.0{approx}6.0 Gy, r were -0.99 and -0.98, the D{sub 0} were 5.88 and 7.36 Gy respectively. The declining tendency in colonies formed by BL-CFC was the same as that of TL-CFC, r = -0.97, for the range of 0{approx}1.0 Gy, r = -0.97, for the range of 1.0{approx}3.0, the D{sub 0} values were 1.35 and 4.36 Gy respectively. The results from these experiments shown that the colony technique was a good method for the study in radiosensitivity.}
place = {China}
year = {1991}
month = {Jul}
}