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Study on labelling conditions of {sup 125}I-SOD chloramine T method and Iodogen method

Technical Report:

Abstract

The five factors (i.e. labelling temperature, chloramine T quantity, labelling reaction time, concentration and volume of PB buffer solution) effecting the labelling of {sup 125}I-SOD chloramine T method were studied by an orthogonal experimental table. Each factor had four kinds of level. The labelled product was separated by chromatographic column Sephadex G25, then the labelling rate and relative activity were determined. An experiment of sixteen groups was enough for obtaining optimal labelling conditions. On the basis of study on chloramine T method, the three factors (i.e. labelling temperature, Iodogen quantity and reaction time) effecting the labelling of {sup 125}I-SOD Iodogen method were also completed by another orthogonal experimental table, and three kinds of label were adopted for each factor. The optimal conditions were reached after nine groups of experiment have been completed. The final results showed that when the labelling temperature was 15 deg C and reaction time was 15 minutes, for 20 {mu}g SOD the Iodogen was 50 {mu}g, and the labelling rate was 79.0%, {sup 125}I-SOD relative activity was 98.2%, radiochemical purity of {sup 125}I-SOD was 95.7%, specific radioactivity was 2.92 x 10{sup 5} Bq`{mu}g. The Iodogen method is much better than chloramine T method.
Authors:
Yizhong, Qiang; Weiming, Shen; Jiagui, Jiang; Hongde, Yang [1] 
  1. Suzhou Medical Coll. (China)
Publication Date:
Sep 01, 1991
Product Type:
Technical Report
Report Number:
CNIC-00507; SMC-0062.
Reference Number:
SCA: 400703; PA: AIX-24:004992; SN: 93000930231
Resource Relation:
Other Information: PBD: Sep 1991
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; OXIDOREDUCTASES; LABELLING; ACTIVITY LEVELS; CHLORAMINES; IMPURITIES; IODINE 125; LABELLED COMPOUNDS; TEMPERATURE DEPENDENCE; TIME DEPENDENCE; 400703; RADIOISOTOPE PRODUCTION
OSTI ID:
10118661
Research Organizations:
China Nuclear Information Centre, Beijing, BJ (China)
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Other: ON: DE93610799; ISBN 7-5022-0499-7; TRN: CN9201676004992
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
[9] p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Yizhong, Qiang, Weiming, Shen, Jiagui, Jiang, and Hongde, Yang. Study on labelling conditions of {sup 125}I-SOD chloramine T method and Iodogen method. China: N. p., 1991. Web.
Yizhong, Qiang, Weiming, Shen, Jiagui, Jiang, & Hongde, Yang. Study on labelling conditions of {sup 125}I-SOD chloramine T method and Iodogen method. China.
Yizhong, Qiang, Weiming, Shen, Jiagui, Jiang, and Hongde, Yang. 1991. "Study on labelling conditions of {sup 125}I-SOD chloramine T method and Iodogen method." China.
@misc{etde_10118661,
title = {Study on labelling conditions of {sup 125}I-SOD chloramine T method and Iodogen method}
author = {Yizhong, Qiang, Weiming, Shen, Jiagui, Jiang, and Hongde, Yang}
abstractNote = {The five factors (i.e. labelling temperature, chloramine T quantity, labelling reaction time, concentration and volume of PB buffer solution) effecting the labelling of {sup 125}I-SOD chloramine T method were studied by an orthogonal experimental table. Each factor had four kinds of level. The labelled product was separated by chromatographic column Sephadex G25, then the labelling rate and relative activity were determined. An experiment of sixteen groups was enough for obtaining optimal labelling conditions. On the basis of study on chloramine T method, the three factors (i.e. labelling temperature, Iodogen quantity and reaction time) effecting the labelling of {sup 125}I-SOD Iodogen method were also completed by another orthogonal experimental table, and three kinds of label were adopted for each factor. The optimal conditions were reached after nine groups of experiment have been completed. The final results showed that when the labelling temperature was 15 deg C and reaction time was 15 minutes, for 20 {mu}g SOD the Iodogen was 50 {mu}g, and the labelling rate was 79.0%, {sup 125}I-SOD relative activity was 98.2%, radiochemical purity of {sup 125}I-SOD was 95.7%, specific radioactivity was 2.92 x 10{sup 5} Bq`{mu}g. The Iodogen method is much better than chloramine T method.}
place = {China}
year = {1991}
month = {Sep}
}