Abstract
Escherichia coli, as many other organisms, has genetic repair mechanisms that increase the survival when its genetic material has been damaged. A consequence of such mechanisms is known as the indirect recombinogenesis of lambda phage. It appears when this phage multiplies into a damaged host bacterium and consists of a stimulation in recombination processes between different viral chromosomes. There are no evidences about the origin of such stimulation, but it seems that RecBCD enzyme from E. coli is necessary for this phenomenon to take place. In this work the role of the RecBCD double-strand DNA exonuclease activity (specified by RecD sub unity of the enzyme) has been studied, to determine if this enzymatic activity is required for the indirect recombinogenesis of lambda phage and with this purpose, recD1013 mutants of E. coli were used as hosts in lambda phage crosses. recD1013 mutants are deficient in the double-stranded DNA exonuclease activity of RecBCD but normal in their recombination and DNA repair abilities, seemingly thanks to the presence of a functional recJ gene, whose product is a double-strand DNA exonuclease too. The results show that the indirect recombinogenesis of lambda needs a functional recD gene and so the RecBCD exonuclease activity should
More>>
Citation Formats
Valdes, M J.
Influence of recD1013 and recJ284 mutations of Escherichia coli on indirect recombinogenesis of lambda phage.; Influencia de las mutaciones recD1013 y recJ284 de Escherichia coli sobre la recombinogenesis indirecta de lambda..
Mexico: N. p.,
1994.
Web.
Valdes, M J.
Influence of recD1013 and recJ284 mutations of Escherichia coli on indirect recombinogenesis of lambda phage.; Influencia de las mutaciones recD1013 y recJ284 de Escherichia coli sobre la recombinogenesis indirecta de lambda..
Mexico.
Valdes, M J.
1994.
"Influence of recD1013 and recJ284 mutations of Escherichia coli on indirect recombinogenesis of lambda phage.; Influencia de las mutaciones recD1013 y recJ284 de Escherichia coli sobre la recombinogenesis indirecta de lambda."
Mexico.
@misc{etde_10115112,
title = {Influence of recD1013 and recJ284 mutations of Escherichia coli on indirect recombinogenesis of lambda phage.; Influencia de las mutaciones recD1013 y recJ284 de Escherichia coli sobre la recombinogenesis indirecta de lambda.}
author = {Valdes, M J}
abstractNote = {Escherichia coli, as many other organisms, has genetic repair mechanisms that increase the survival when its genetic material has been damaged. A consequence of such mechanisms is known as the indirect recombinogenesis of lambda phage. It appears when this phage multiplies into a damaged host bacterium and consists of a stimulation in recombination processes between different viral chromosomes. There are no evidences about the origin of such stimulation, but it seems that RecBCD enzyme from E. coli is necessary for this phenomenon to take place. In this work the role of the RecBCD double-strand DNA exonuclease activity (specified by RecD sub unity of the enzyme) has been studied, to determine if this enzymatic activity is required for the indirect recombinogenesis of lambda phage and with this purpose, recD1013 mutants of E. coli were used as hosts in lambda phage crosses. recD1013 mutants are deficient in the double-stranded DNA exonuclease activity of RecBCD but normal in their recombination and DNA repair abilities, seemingly thanks to the presence of a functional recJ gene, whose product is a double-strand DNA exonuclease too. The results show that the indirect recombinogenesis of lambda needs a functional recD gene and so the RecBCD exonuclease activity should be essential for the event. However, they do not allow to establish if this activity is enough or some other of the multiple activities of the enzyme are required. Since RecBCD is inhibited by lambda-Gamm protein during the lytic growth of the phage, there should be some way to counteract the inhibition made by this protein, unless the concentration reached by Gamm in infected cells is too low to suppress completely the action of the enzyme RecBCD. The results too show that in our experimental conditions, the exonuclease specified by RecBCD cannot be substituted by the activity of the recJ gene product in the indirect recombinogenesis of lambda.}
place = {Mexico}
year = {1994}
month = {Dec}
}
title = {Influence of recD1013 and recJ284 mutations of Escherichia coli on indirect recombinogenesis of lambda phage.; Influencia de las mutaciones recD1013 y recJ284 de Escherichia coli sobre la recombinogenesis indirecta de lambda.}
author = {Valdes, M J}
abstractNote = {Escherichia coli, as many other organisms, has genetic repair mechanisms that increase the survival when its genetic material has been damaged. A consequence of such mechanisms is known as the indirect recombinogenesis of lambda phage. It appears when this phage multiplies into a damaged host bacterium and consists of a stimulation in recombination processes between different viral chromosomes. There are no evidences about the origin of such stimulation, but it seems that RecBCD enzyme from E. coli is necessary for this phenomenon to take place. In this work the role of the RecBCD double-strand DNA exonuclease activity (specified by RecD sub unity of the enzyme) has been studied, to determine if this enzymatic activity is required for the indirect recombinogenesis of lambda phage and with this purpose, recD1013 mutants of E. coli were used as hosts in lambda phage crosses. recD1013 mutants are deficient in the double-stranded DNA exonuclease activity of RecBCD but normal in their recombination and DNA repair abilities, seemingly thanks to the presence of a functional recJ gene, whose product is a double-strand DNA exonuclease too. The results show that the indirect recombinogenesis of lambda needs a functional recD gene and so the RecBCD exonuclease activity should be essential for the event. However, they do not allow to establish if this activity is enough or some other of the multiple activities of the enzyme are required. Since RecBCD is inhibited by lambda-Gamm protein during the lytic growth of the phage, there should be some way to counteract the inhibition made by this protein, unless the concentration reached by Gamm in infected cells is too low to suppress completely the action of the enzyme RecBCD. The results too show that in our experimental conditions, the exonuclease specified by RecBCD cannot be substituted by the activity of the recJ gene product in the indirect recombinogenesis of lambda.}
place = {Mexico}
year = {1994}
month = {Dec}
}