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A new technique for ion beam tritium labelling

Technical Report:

Abstract

An advanced technique, the ion beam tritium labelling method (IBTL), used for labelling proteins, peptides and other nonvolatile organic compounds is introduced. In this method the excited tritium ion beam is accelerated and then bombs a solid sample target in which tritium exchanging for hydrogen is taken place. The IBTL has been used for preparation of tritiated soybean trypsin inhibitor, ribonuclease A, elastin and pachyman etc. After purifing by dialysis, ion exchange chromatography and gel filtration, the tritiated proteins and polysaccharide were obtained with the specific activity over 37 GBq/mmol, the function of tritiated decomposition products was not found. The product was shown to have native biological activity. Amino acid analysis of tritiated protein showed that the relative specific radioactivities were higher for His., Tyr. and Phe. but lower for Val., Ile. and Ser.
Authors:
Nianbao, Zhang; Shugang, Sheng; Fuzeng, Yao [1] 
  1. Academia Sinica, Shanghai, SH (China). Shanghai Inst. of Nuclear Research; and others
Publication Date:
Jun 01, 1990
Product Type:
Technical Report
Report Number:
CNIC-00410; SINR-0001.
Reference Number:
SCA: 400703; PA: AIX-23:010631; SN: 92000637582
Resource Relation:
Other Information: PBD: Jun 1990
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; ION BEAMS; LABELLING; TRITIUM; PEPTIDES; POLYSACCHARIDES; PROTEINS; RNA-ASE; TRYPSIN; 400703; RADIOISOTOPE PRODUCTION
OSTI ID:
10111932
Research Organizations:
China Nuclear Information Centre, Beijing, BJ (China)
Country of Origin:
China
Language:
Chinese
Other Identifying Numbers:
Other: ON: DE92613351; TRN: CN9101687010631
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
9 p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Nianbao, Zhang, Shugang, Sheng, and Fuzeng, Yao. A new technique for ion beam tritium labelling. China: N. p., 1990. Web.
Nianbao, Zhang, Shugang, Sheng, & Fuzeng, Yao. A new technique for ion beam tritium labelling. China.
Nianbao, Zhang, Shugang, Sheng, and Fuzeng, Yao. 1990. "A new technique for ion beam tritium labelling." China.
@misc{etde_10111932,
title = {A new technique for ion beam tritium labelling}
author = {Nianbao, Zhang, Shugang, Sheng, and Fuzeng, Yao}
abstractNote = {An advanced technique, the ion beam tritium labelling method (IBTL), used for labelling proteins, peptides and other nonvolatile organic compounds is introduced. In this method the excited tritium ion beam is accelerated and then bombs a solid sample target in which tritium exchanging for hydrogen is taken place. The IBTL has been used for preparation of tritiated soybean trypsin inhibitor, ribonuclease A, elastin and pachyman etc. After purifing by dialysis, ion exchange chromatography and gel filtration, the tritiated proteins and polysaccharide were obtained with the specific activity over 37 GBq/mmol, the function of tritiated decomposition products was not found. The product was shown to have native biological activity. Amino acid analysis of tritiated protein showed that the relative specific radioactivities were higher for His., Tyr. and Phe. but lower for Val., Ile. and Ser.}
place = {China}
year = {1990}
month = {Jun}
}