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Production of lyophilized macroaggregated albumin (MAA) kits to be labelled with sup(99m)Tc for lung scanning; Producao de conjuntos de reativos de macroagregado de soro albumina humano (MAA) para marcacao com sup(99m)Tc destinado a cintilografia pulmonar

Technical Report:

Abstract

Among the various macroaggregation procedures described in the literature, we have developed a rapid and reliable method for the routine production of lyophilized Sn-Macroaggregated Albumin (Sn-MAA) kits to be labelled with {sup 99m}Tc for lung perfusion scanning. The reaction vial contains a lyophilized suspension having the following components: 1,0mg of Aggregated Human Serum Albumin (MAA), 0,12mg of hydrated stannous chloride (SnCl{sub 2}.2H{sub 2}O), 5,0mg of Normal Human Serum Albumin (HSA) and 1% of Tween 80 as emulsifying agent. In this method the Sn-MAA kits were performed in a single step process, and a consistent suitable range of particle size (10-80 mu) was obtained. The size of the particles and the effect of storage time on the stability for the lyophilized kits were also investigated. The radiochemical purity, higher than 98% in {sup 99m}Tc Macroaggregated was determined by using ascending paper chromatography (Whatman n.3 and 85% methanol): biodistribution studies in rats have shown the high uptake in the lungs 85%/D with low liver uptake 2%/d and lung-liver ratios 98 {+-} 1%. Excellent human lung images for clinical practice were obtained with these kits, which are prepared at IPEN-CNEN/SP for brazilian physicians. (author).
Publication Date:
Jun 01, 1991
Product Type:
Technical Report
Report Number:
IPEN-PUB-338
Reference Number:
SCA: 400703; PA: AIX-23:010632; SN: 92000637583
Resource Relation:
Other Information: PBD: Jun 1991
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; ALBUMINS; LUNGS; PRODUCTION; TECHNETIUM 99 TARGET; LYOPHILIZATION; SCINTISCANNING; 400703; RADIOISOTOPE PRODUCTION
OSTI ID:
10111322
Research Organizations:
Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)
Country of Origin:
Brazil
Language:
Portuguese
Other Identifying Numbers:
Other: ON: DE92613352; TRN: BR9228284010632
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
14 p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Hamada, E S, Muramoto, E, Brito, R H, Sosa de Pereira, N P, and Almeida, M A.T. de. Production of lyophilized macroaggregated albumin (MAA) kits to be labelled with sup(99m)Tc for lung scanning; Producao de conjuntos de reativos de macroagregado de soro albumina humano (MAA) para marcacao com sup(99m)Tc destinado a cintilografia pulmonar. Brazil: N. p., 1991. Web.
Hamada, E S, Muramoto, E, Brito, R H, Sosa de Pereira, N P, & Almeida, M A.T. de. Production of lyophilized macroaggregated albumin (MAA) kits to be labelled with sup(99m)Tc for lung scanning; Producao de conjuntos de reativos de macroagregado de soro albumina humano (MAA) para marcacao com sup(99m)Tc destinado a cintilografia pulmonar. Brazil.
Hamada, E S, Muramoto, E, Brito, R H, Sosa de Pereira, N P, and Almeida, M A.T. de. 1991. "Production of lyophilized macroaggregated albumin (MAA) kits to be labelled with sup(99m)Tc for lung scanning; Producao de conjuntos de reativos de macroagregado de soro albumina humano (MAA) para marcacao com sup(99m)Tc destinado a cintilografia pulmonar." Brazil.
@misc{etde_10111322,
title = {Production of lyophilized macroaggregated albumin (MAA) kits to be labelled with sup(99m)Tc for lung scanning; Producao de conjuntos de reativos de macroagregado de soro albumina humano (MAA) para marcacao com sup(99m)Tc destinado a cintilografia pulmonar}
author = {Hamada, E S, Muramoto, E, Brito, R H, Sosa de Pereira, N P, and Almeida, M A.T. de}
abstractNote = {Among the various macroaggregation procedures described in the literature, we have developed a rapid and reliable method for the routine production of lyophilized Sn-Macroaggregated Albumin (Sn-MAA) kits to be labelled with {sup 99m}Tc for lung perfusion scanning. The reaction vial contains a lyophilized suspension having the following components: 1,0mg of Aggregated Human Serum Albumin (MAA), 0,12mg of hydrated stannous chloride (SnCl{sub 2}.2H{sub 2}O), 5,0mg of Normal Human Serum Albumin (HSA) and 1% of Tween 80 as emulsifying agent. In this method the Sn-MAA kits were performed in a single step process, and a consistent suitable range of particle size (10-80 mu) was obtained. The size of the particles and the effect of storage time on the stability for the lyophilized kits were also investigated. The radiochemical purity, higher than 98% in {sup 99m}Tc Macroaggregated was determined by using ascending paper chromatography (Whatman n.3 and 85% methanol): biodistribution studies in rats have shown the high uptake in the lungs 85%/D with low liver uptake 2%/d and lung-liver ratios 98 {+-} 1%. Excellent human lung images for clinical practice were obtained with these kits, which are prepared at IPEN-CNEN/SP for brazilian physicians. (author).}
place = {Brazil}
year = {1991}
month = {Jun}
}