A first complete integrated test of the J-FCU (JAERI Fuel Cleanup System) and the ISS (Isotope Separation System) with full four cryogenic distillation columns was performed successfully with 100 grams of tritium. This test was performed as a part of a 25 day TSTA extended loop run on April - May 1992. Through this operation, the J-FCU operated stably and safely. No significant problems was caused on the interaction between the J-FCU and the ISS. J-FCU processed more than 5 N1/min of simulated fusion-fuel exhaust with wide range of impurities and transferred pure hydrogen isotopes to the ISS and exhausted tritium free impurities to the TWT (Tritium Waste Treatment System) continuously. This report describes the detail result of the J-FCU part test under a 25 day TSTA extended loop run and discuss the JFCU performance. (author).