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Determination of methyl methanesulfonate pretreatment effect in Drosophila melanogaster larvaes upon repair mechanisms in somatic cells.; Determinar el efecto del pretratamiento del metil metanosulfonato en larvas de Drosophila melanogaster sobre los mecanismos de reparacion en celulas somaticas.

Abstract

To make evident the existence of SOS repair mecanism in somatic cells, larvaes of drosophila melanogaster with MWH markers for females and FLR markers for males were used. This larvaes received a pretreatment with MMS at concentrations of 0.0007% and 0.0014% during 24 hours and latter a treatment with gamma rays at different dosis. SMART program was used to make stastistical evaluations. Small spots were observed which can have two origins. First could be damage in the last part of third stage in which cells are in last divisions and second could be the damage to larvaes in early stages in shich pretreatment with MMS cause lesions which prevent the reproduction of the cells. Also big spots were observed which presence is due to recombination. It was detected than the bigger the concentration of MMS and radiation dose, the bigger the induced damage. In some groups such observation was impossible may be to technical problems as relative humidity, out of phase in the growth of larvaes giving place that treatment were given in three stages. For this reasons it was impossible to discriminate if drosophila melanogaster is wheter or not capable to induce a repair mechanism (Author).
Authors:
Hernandez Paz, M [1] 
  1. Escuela de Biologia, Instituto de Ciencias y Artes de Chiapas (Mexico)
Publication Date:
Dec 31, 1992
Product Type:
Thesis/Dissertation
Report Number:
INIS-mf-13395
Reference Number:
SCA: 560152; PA: AIX-24:000898; SN: 93000912929
Resource Relation:
Other Information: TH: Thesis (Biologist).; PBD: 1992
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DROSOPHILA; METHYL METHANESULFONATE; LARVAE; BIOLOGICAL REPAIR; DNA REPAIR; GAMMA RADIATION; RECOMBINANT DNA; S CODES; SOMATIC CELLS; STATISTICS; 560152; ANIMALS
OSTI ID:
10108495
Research Organizations:
Instituto de Ciencias y Artes, Chiapas (Mexico). Escuela de Biologia
Country of Origin:
Mexico
Language:
Spanish
Other Identifying Numbers:
Other: ON: DE93609142; TRN: MX9200101000898
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
[76] p.
Announcement Date:
Jun 30, 2005

Citation Formats

Hernandez Paz, M. Determination of methyl methanesulfonate pretreatment effect in Drosophila melanogaster larvaes upon repair mechanisms in somatic cells.; Determinar el efecto del pretratamiento del metil metanosulfonato en larvas de Drosophila melanogaster sobre los mecanismos de reparacion en celulas somaticas.. Mexico: N. p., 1992. Web.
Hernandez Paz, M. Determination of methyl methanesulfonate pretreatment effect in Drosophila melanogaster larvaes upon repair mechanisms in somatic cells.; Determinar el efecto del pretratamiento del metil metanosulfonato en larvas de Drosophila melanogaster sobre los mecanismos de reparacion en celulas somaticas.. Mexico.
Hernandez Paz, M. 1992. "Determination of methyl methanesulfonate pretreatment effect in Drosophila melanogaster larvaes upon repair mechanisms in somatic cells.; Determinar el efecto del pretratamiento del metil metanosulfonato en larvas de Drosophila melanogaster sobre los mecanismos de reparacion en celulas somaticas." Mexico.
@misc{etde_10108495,
title = {Determination of methyl methanesulfonate pretreatment effect in Drosophila melanogaster larvaes upon repair mechanisms in somatic cells.; Determinar el efecto del pretratamiento del metil metanosulfonato en larvas de Drosophila melanogaster sobre los mecanismos de reparacion en celulas somaticas.}
author = {Hernandez Paz, M}
abstractNote = {To make evident the existence of SOS repair mecanism in somatic cells, larvaes of drosophila melanogaster with MWH markers for females and FLR markers for males were used. This larvaes received a pretreatment with MMS at concentrations of 0.0007% and 0.0014% during 24 hours and latter a treatment with gamma rays at different dosis. SMART program was used to make stastistical evaluations. Small spots were observed which can have two origins. First could be damage in the last part of third stage in which cells are in last divisions and second could be the damage to larvaes in early stages in shich pretreatment with MMS cause lesions which prevent the reproduction of the cells. Also big spots were observed which presence is due to recombination. It was detected than the bigger the concentration of MMS and radiation dose, the bigger the induced damage. In some groups such observation was impossible may be to technical problems as relative humidity, out of phase in the growth of larvaes giving place that treatment were given in three stages. For this reasons it was impossible to discriminate if drosophila melanogaster is wheter or not capable to induce a repair mechanism (Author).}
place = {Mexico}
year = {1992}
month = {Dec}
}