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Laboratory soft x-ray microscopy and tomography

Technical Report:

Abstract

Soft x-ray microscopy in the water-window (lambda = 2.28 nm - 4.36 nm) is based on zone-plate optics and allows high-resolution imaging of, e.g., cells and soils in their natural or near-natural environment. Three-dimensional imaging is provided via tomographic techniques, soft x-ray cryo tomography. However, soft x-ray microscopes with such capabilities have been based on large-scale synchrotron x-ray facilities, thereby limiting their accessibility for a wider scientific community. This Thesis describes the development of the Stockholm laboratory soft x-ray microscope to three-dimensional cryo tomography and to new optics-based contrast mechanisms. The microscope relies on a methanol or nitrogen liquid-jet laser-plasma source, normal-incidence multilayer or zone-plate condenser optics, in-house fabricated zone-plate objectives, and allows operation at two wavelengths in the water-window, lambda = 2.48 nm and lambda = 2.48 nm. With the implementation of a new state-of-the-art normal-incidence multilayer condenser for operation at lambda = 2.48 nm and a tiltable cryogenic sample stage the microscope now allows imaging of dry, wet or cryo-fixed samples. This arrangement was used for the first demonstration of laboratory soft x-ray cryo microscopy and tomography. The performance of the microscope has been demonstrated in a number of experiments described in this Thesis, including, tomographic imaging with  More>>
Publication Date:
Jul 01, 2011
Product Type:
Technical Report
Report Number:
TRITA-FYS-2011-03
Resource Relation:
Other Information: Thesis or Dissertation; TH: Doctoral thesis (TeknD); 175 refs., figs, tabs.
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; MICROSCOPY; OPTICS; PLATES; TOMOGRAPHY; X RADIATION
OSTI ID:
1010817
Research Organizations:
KTH Royal Inst. of Technology, Stockholm (Sweden). School of Engineering Sciences, Applied Physics, Biomedical and X-ray Physics
Country of Origin:
Sweden
Language:
English
Other Identifying Numbers:
Other: ISBN 978-91-7415-874-8; ISSN 0280-316X; TRN: SE1108061
Availability:
Available from: http://urn.kb.se/resolve?urn=urn:nbn:se:kth:diva-29950
Submitting Site:
SWDN
Size:
76 p. pages
Announcement Date:
Apr 11, 2011

Technical Report:

Citation Formats

Bertilson, Michael. Laboratory soft x-ray microscopy and tomography. Sweden: N. p., 2011. Web.
Bertilson, Michael. Laboratory soft x-ray microscopy and tomography. Sweden.
Bertilson, Michael. 2011. "Laboratory soft x-ray microscopy and tomography." Sweden.
@misc{etde_1010817,
title = {Laboratory soft x-ray microscopy and tomography}
author = {Bertilson, Michael}
abstractNote = {Soft x-ray microscopy in the water-window (lambda = 2.28 nm - 4.36 nm) is based on zone-plate optics and allows high-resolution imaging of, e.g., cells and soils in their natural or near-natural environment. Three-dimensional imaging is provided via tomographic techniques, soft x-ray cryo tomography. However, soft x-ray microscopes with such capabilities have been based on large-scale synchrotron x-ray facilities, thereby limiting their accessibility for a wider scientific community. This Thesis describes the development of the Stockholm laboratory soft x-ray microscope to three-dimensional cryo tomography and to new optics-based contrast mechanisms. The microscope relies on a methanol or nitrogen liquid-jet laser-plasma source, normal-incidence multilayer or zone-plate condenser optics, in-house fabricated zone-plate objectives, and allows operation at two wavelengths in the water-window, lambda = 2.48 nm and lambda = 2.48 nm. With the implementation of a new state-of-the-art normal-incidence multilayer condenser for operation at lambda = 2.48 nm and a tiltable cryogenic sample stage the microscope now allows imaging of dry, wet or cryo-fixed samples. This arrangement was used for the first demonstration of laboratory soft x-ray cryo microscopy and tomography. The performance of the microscope has been demonstrated in a number of experiments described in this Thesis, including, tomographic imaging with a resolution of 140 nm, cryo microscopy and tomography of various cells and parasites, and for studies of aqueous soils and clays. The Thesis also describes the development and implementation of single-element differential-interference and Zernike phase-contrast zone-plate objectives. The enhanced contrast provided by these optics reduce exposure times or lowers the dose in samples and are of major importance for harder x-ray microscopy. The implementation of a high-resolution 50 nm compound zone-plate objective for sub-25-nm resolution imaging is also described. All experiments are supported by extensive numerical modelling for improved understanding of partially coherent image formation and stray light in soft x-ray microscopes. The models are useful tools for studying effects of zone plate optics or optical design of the microscope on image formation and quantitative accuracy in soft x-ray tomography}
place = {Sweden}
year = {2011}
month = {Jul}
}