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Optimization of the synthesis of a high specific activity ``125 I-labelled hapten for radioimmunoassays; Optimizacion de un metodo de preparacion de un radiohapteno -``125 I para ria de alta actividad especifica

Abstract

In this first report it is described the synthesis, separation and purification of the 2-radioiodinated histamine ``125 I-labelled histamine by a mixed anhydride reaction. About 75% incorporation of I``1125, from Na``125, I, was achieved with a molecular ratio of 1:1 mixed anhydride:histamine. The radiochemical purity of the conjugate by TLC was >99% and its theoretical specific activity, 3850 mu Ci/mug. Dissolved in ethanol and held at -20 degree centigree under darkness decomposition on storage did not exceed 1% per month.
Publication Date:
Dec 01, 1994
Product Type:
Technical Report
Report Number:
CIEMAT-734
Reference Number:
SCA: 400703; 550601; PA: SPN-94:0B0783; EDB-95:016215; SN: 94001295718
Resource Relation:
Other Information: PBD: [1994]
Subject:
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY; 62 RADIOLOGY AND NUCLEAR MEDICINE; HISTAMINE; LABELLING; RADIOCHEMISTRY; RADIOIMMUNOASSAY; AMINES; ANHYDRIDES; ETHANOL; IODINE 125; 400703; 550601; RADIOISOTOPE PRODUCTION; UNSEALED RADIONUCLIDES IN DIAGNOSTICS
OSTI ID:
10104672
Research Organizations:
Centro de Investigaciones Energeticas, Medioambientales y Tecnologicas (CIEMAT), Madrid (Spain)
Country of Origin:
Spain
Language:
Spanish
Other Identifying Numbers:
Other: ON: DE95721641; TRN: ES94B0783
Availability:
OSTI; NTIS; INIS
Submitting Site:
INIS
Size:
17 p.
Announcement Date:
Jun 30, 2005

Citation Formats

Suarez, C, Simon, M A, Paz, D, and Romero del Hombrebueno, B. Optimization of the synthesis of a high specific activity ``125 I-labelled hapten for radioimmunoassays; Optimizacion de un metodo de preparacion de un radiohapteno -``125 I para ria de alta actividad especifica. Spain: N. p., 1994. Web.
Suarez, C, Simon, M A, Paz, D, & Romero del Hombrebueno, B. Optimization of the synthesis of a high specific activity ``125 I-labelled hapten for radioimmunoassays; Optimizacion de un metodo de preparacion de un radiohapteno -``125 I para ria de alta actividad especifica. Spain.
Suarez, C, Simon, M A, Paz, D, and Romero del Hombrebueno, B. 1994. "Optimization of the synthesis of a high specific activity ``125 I-labelled hapten for radioimmunoassays; Optimizacion de un metodo de preparacion de un radiohapteno -``125 I para ria de alta actividad especifica." Spain.
@misc{etde_10104672,
title = {Optimization of the synthesis of a high specific activity ``125 I-labelled hapten for radioimmunoassays; Optimizacion de un metodo de preparacion de un radiohapteno -``125 I para ria de alta actividad especifica}
author = {Suarez, C, Simon, M A, Paz, D, and Romero del Hombrebueno, B}
abstractNote = {In this first report it is described the synthesis, separation and purification of the 2-radioiodinated histamine ``125 I-labelled histamine by a mixed anhydride reaction. About 75% incorporation of I``1125, from Na``125, I, was achieved with a molecular ratio of 1:1 mixed anhydride:histamine. The radiochemical purity of the conjugate by TLC was >99% and its theoretical specific activity, 3850 mu Ci/mug. Dissolved in ethanol and held at -20 degree centigree under darkness decomposition on storage did not exceed 1% per month.}
place = {Spain}
year = {1994}
month = {Dec}
}