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Effects of ultraviolet radiation on microtubule organisation and morphogenesis in plants

Thesis/Dissertation:

Abstract

The involvement of the cytoskeleton in the development of somatic embryos was studied in Larix x eurolepis. Protoplasts were isolated from both somatic embryo-regenerating and non-generating cultures and fractionated on a discontinuous Percoll density gradient. Protoplasts of two cell lines of Larix eurolepis, one with regenerating potential and one lacking this potential, were compared. In contrast to the non-regenerating line were a protoplast-like organisation of the cortical microtubules was maintained, re-organisation of this microtubular network occurred in the regenerable line after only three days of culture, indicating that organised growth was occurring. However, this early organisation of cortical microtubules may not always be a valid marker for regenerable and non-regenerable material. In order to investigate the effect of ultraviolet-B (UV-B, 280-320 nm) radiation on the microtubule cytoskeleton, protoplasts were isolated from leaves of Petunia hybrida and subjected to four different doses of UV-B radiation. The organisation of the microtubules and the progression of the cells through the cell cycle was observed at 0, 24, 48 and 72 h after irradiation. UV-B induced breaks in the cortical microtubules resulting in shorter fragments with increasing amounts of radiation. Also, the division of the protoplasts was delayed. Whole Petunia plants were grown in  More>>
Authors:
Publication Date:
Sep 01, 1994
Product Type:
Thesis/Dissertation
Report Number:
LUNBDS-NBFB-1028/1-88(1994)
Reference Number:
SCA: 560120; PA: AIX-26:005459; EDB-95:010307; SN: 95001298998
Resource Relation:
Other Information: TH: Doctoral Diss. (FD).; PBD: Sep 1994
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; PLANT CELLS; MORPHOLOGICAL CHANGES; ULTRAVIOLET RADIATION; BIOLOGICAL RADIATION EFFECTS; CARBON DIOXIDE; MICROTUBULES; MORPHOLOGY; OZONE; PLANTS; UPTAKE; 560120; RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
OSTI ID:
10103665
Research Organizations:
Lund Univ. (Sweden). Dept. of Plant Physiology
Country of Origin:
Sweden
Language:
English
Other Identifying Numbers:
Other: ON: DE95609825; ISBN 91-628-1345-5; TRN: SE9400177005459
Availability:
OSTI; NTIS; INIS
Submitting Site:
SWDN
Size:
44 p.
Announcement Date:
Jun 30, 2005

Thesis/Dissertation:

Citation Formats

Staxen, I. Effects of ultraviolet radiation on microtubule organisation and morphogenesis in plants. Sweden: N. p., 1994. Web.
Staxen, I. Effects of ultraviolet radiation on microtubule organisation and morphogenesis in plants. Sweden.
Staxen, I. 1994. "Effects of ultraviolet radiation on microtubule organisation and morphogenesis in plants." Sweden.
@misc{etde_10103665,
title = {Effects of ultraviolet radiation on microtubule organisation and morphogenesis in plants}
author = {Staxen, I}
abstractNote = {The involvement of the cytoskeleton in the development of somatic embryos was studied in Larix x eurolepis. Protoplasts were isolated from both somatic embryo-regenerating and non-generating cultures and fractionated on a discontinuous Percoll density gradient. Protoplasts of two cell lines of Larix eurolepis, one with regenerating potential and one lacking this potential, were compared. In contrast to the non-regenerating line were a protoplast-like organisation of the cortical microtubules was maintained, re-organisation of this microtubular network occurred in the regenerable line after only three days of culture, indicating that organised growth was occurring. However, this early organisation of cortical microtubules may not always be a valid marker for regenerable and non-regenerable material. In order to investigate the effect of ultraviolet-B (UV-B, 280-320 nm) radiation on the microtubule cytoskeleton, protoplasts were isolated from leaves of Petunia hybrida and subjected to four different doses of UV-B radiation. The organisation of the microtubules and the progression of the cells through the cell cycle was observed at 0, 24, 48 and 72 h after irradiation. UV-B induced breaks in the cortical microtubules resulting in shorter fragments with increasing amounts of radiation. Also, the division of the protoplasts was delayed. Whole Petunia plants were grown in growth chambers in the presence and absence of UV-B. The plants responded to UV-B with increased rates of CO{sub 2} assimilation, a 60% increase in UV-screening compounds and the changes in the morphology of the leaves that were reflected in a 70-100% increase in leaf area and 20% decrease in leaf thickness. The microtubules of the epidermal cells was not affected by UV-B, nor was the number of epidermal cells (per unit area). The increase in leaf area in the UV-treated plants appeared due to stimulation of cell division in the leaf meristems. 111 refs, 5 figs, 2 tabs.}
place = {Sweden}
year = {1994}
month = {Sep}
}