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Radioprotective effects of dimethyl sulfoxide in two biological systems

Technical Report:

Abstract

The present study was conducted: a) to confirm data from others describing a radioprotective capacity of DMSO in mice; b) to establish whether this protective behavior could be evidenced in an in vitro chemical system utilizing bovine crystalline protein as target. Heterozigous female albino mice were used for the 30-day-survival studies after 9 Gy sup(60)Co gamma irradiation (dose rate: 4.5 Gy/min) injected 1 h prior with 2000 mg/kg DMSO intraperitoneally. Total body weight curves during the same period were also analysed. For the molecular level studies 1 M DMSO was added to a series of aqueous protein solutions from bovine lens and 10 min later irradiated with 5 different doses from 5,000 to 25,000 Gy sup(60)Co (average dose rate 14 Gy/min). After irradiation, spectrophotometric reading at 600 nm and free thiol group determinations were performed in order to evaluate the radiation-induced modifications. (author).
Publication Date:
Mar 01, 1991
Product Type:
Technical Report
Report Number:
IPEN-PUB-332
Reference Number:
SCA: 560120; PA: AIX-23:004884; SN: 91000615320
Resource Relation:
Other Information: PBD: Mar 1991
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DMSO; RADIOPROTECTIVE SUBSTANCES; IN VITRO; IN VIVO; IRRADIATION; 560120; RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
OSTI ID:
10103324
Research Organizations:
Instituto de Pesquisas Energeticas e Nucleares (IPEN), Sao Paulo, SP (Brazil)
Country of Origin:
Brazil
Language:
English
Other Identifying Numbers:
Other: ON: DE92611579; TRN: BR9127905004884
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
16 p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Mastro, N.L. del, Bernardes, D M.L., and Villavicencio, A L.C. Radioprotective effects of dimethyl sulfoxide in two biological systems. Brazil: N. p., 1991. Web.
Mastro, N.L. del, Bernardes, D M.L., & Villavicencio, A L.C. Radioprotective effects of dimethyl sulfoxide in two biological systems. Brazil.
Mastro, N.L. del, Bernardes, D M.L., and Villavicencio, A L.C. 1991. "Radioprotective effects of dimethyl sulfoxide in two biological systems." Brazil.
@misc{etde_10103324,
title = {Radioprotective effects of dimethyl sulfoxide in two biological systems}
author = {Mastro, N.L. del, Bernardes, D M.L., and Villavicencio, A L.C.}
abstractNote = {The present study was conducted: a) to confirm data from others describing a radioprotective capacity of DMSO in mice; b) to establish whether this protective behavior could be evidenced in an in vitro chemical system utilizing bovine crystalline protein as target. Heterozigous female albino mice were used for the 30-day-survival studies after 9 Gy sup(60)Co gamma irradiation (dose rate: 4.5 Gy/min) injected 1 h prior with 2000 mg/kg DMSO intraperitoneally. Total body weight curves during the same period were also analysed. For the molecular level studies 1 M DMSO was added to a series of aqueous protein solutions from bovine lens and 10 min later irradiated with 5 different doses from 5,000 to 25,000 Gy sup(60)Co (average dose rate 14 Gy/min). After irradiation, spectrophotometric reading at 600 nm and free thiol group determinations were performed in order to evaluate the radiation-induced modifications. (author).}
place = {Brazil}
year = {1991}
month = {Mar}
}