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Cell fusion induced by ionizing radiation in various cell lines

Technical Report:

Abstract

Cell fusion induced by ionizing radiation has been studied in rat`s hepatocytes in vivo and in different cell lines in vitro. These cell lines were: Hela cells, V-79 fibroblasts, human and rat lymphocytes. For irradiation, 0.85 MeV fission neutrons and 14 MeV fast neutrons were used. Cell analyses were performed by fluorescent dyes using immunofluorescent microscope and flow cytometre. Our results in vivo showed that, regardless the dose-rate, a dose of 1 Gy approximately was enough to induce a significant level of cell fusion depending on neutron energy and the age of rats. The level of cell fusion was also significant in Hela cells at a dose of 0.5 Gy. Similar effect, but to a lesser extent, was observed in V-79 cells. Whereas, in lymphocytes insignificant cell fusion was noticed. The varying levels of cell-fusion in different cell lines could be attributed to the type of cells and mutual contact between cells. Furthermore irradiation did not show any influence on cell division ability in both hepatocytes and Hela cells and that fused cells were also able to divide forming a new generation of cells. (author). 36 refs., 8 figs., 10 tabs.
Authors:
Khair, M B [1] 
  1. Atomic Energy Commission, Damascus (Syrian Arab Republic). Dept. of Radiobiology and Health
Publication Date:
Jul 01, 1994
Product Type:
Technical Report
Report Number:
AECS-B/FRSR-84
Reference Number:
SCA: 560120; PA: AIX-26:005452; EDB-95:010308; SN: 95001298992
Resource Relation:
Other Information: PBD: Jul 1994
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; FIBROBLASTS; BIOLOGICAL RADIATION EFFECTS; HELA CELLS; LYMPHOCYTES; ANIMAL CELLS; CELL MEMBRANES; EXPERIMENTAL DATA; FAST NEUTRONS; FISSION NEUTRONS; GAMMA RADIATION; IN VITRO; IN VIVO; IONIZING RADIATIONS; LIVER CELLS; RATS; 560120; RADIATION EFFECTS ON BIOCHEMICALS, CELLS, AND TISSUE CULTURE
OSTI ID:
10102721
Research Organizations:
Atomic Energy Commission, Damascus (Syrian Arab Republic). Dept. of Radiobiology and Health
Country of Origin:
Syrian Arab Republic
Language:
Arabic
Other Identifying Numbers:
Other: ON: DE95609824; TRN: SY9400319005452
Availability:
OSTI; NTIS (US Sales Only); INIS
Submitting Site:
INIS
Size:
41 p.
Announcement Date:
Jun 30, 2005

Technical Report:

Citation Formats

Khair, M B. Cell fusion induced by ionizing radiation in various cell lines. Syrian Arab Republic: N. p., 1994. Web.
Khair, M B. Cell fusion induced by ionizing radiation in various cell lines. Syrian Arab Republic.
Khair, M B. 1994. "Cell fusion induced by ionizing radiation in various cell lines." Syrian Arab Republic.
@misc{etde_10102721,
title = {Cell fusion induced by ionizing radiation in various cell lines}
author = {Khair, M B}
abstractNote = {Cell fusion induced by ionizing radiation has been studied in rat`s hepatocytes in vivo and in different cell lines in vitro. These cell lines were: Hela cells, V-79 fibroblasts, human and rat lymphocytes. For irradiation, 0.85 MeV fission neutrons and 14 MeV fast neutrons were used. Cell analyses were performed by fluorescent dyes using immunofluorescent microscope and flow cytometre. Our results in vivo showed that, regardless the dose-rate, a dose of 1 Gy approximately was enough to induce a significant level of cell fusion depending on neutron energy and the age of rats. The level of cell fusion was also significant in Hela cells at a dose of 0.5 Gy. Similar effect, but to a lesser extent, was observed in V-79 cells. Whereas, in lymphocytes insignificant cell fusion was noticed. The varying levels of cell-fusion in different cell lines could be attributed to the type of cells and mutual contact between cells. Furthermore irradiation did not show any influence on cell division ability in both hepatocytes and Hela cells and that fused cells were also able to divide forming a new generation of cells. (author). 36 refs., 8 figs., 10 tabs.}
place = {Syrian Arab Republic}
year = {1994}
month = {Jul}
}