{
  "date" : "1988-02-11",
  "identifier_doecontract" : "",
  "subject" : "59 BASIC BIOLOGICAL SCIENCES; DNA; CYTOLOGICAL TECHNIQUES; BLOOD CELLS; CELL NUCLEI; DEHYDRATION; HUMAN POPULATIONS; RFLPS; SALTING-OUT AGENTS; SODIUM CHLORIDES; ALKALI METAL COMPOUNDS; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CELL CONSTITUENTS; CHLORIDES; CHLORINE COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; MATERIALS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; POPULATIONS; SODIUM COMPOUNDS; 550200* - Biochemistry",
  "description" : "One of the obstacles encountered when extracting DNA from a large number of samples is the cumbersome method of deproteinizing cell digests with the hazardous organic solvents phenol and isochloroform. A rapid, safe and inexpensive method was developed to simplify the deproteinization procedure. This method involves salting out of the cellular proteins by dehydration and precipitation with a saturated NaCl solution. Buffy coats of nucleated cells obtained from anticoagulated blood (ACD or EDTA) were resuspended with 3 ml of nuclei lysis buffer. The DNA obtained from this simple technique yielded quantities comparable to those obtained from phenol-chloroform extractions. This procedure has been used in our laboratory on several thousand blood samples for parentage, population and forensic studies. This technique is used with out nonisotopic hybridization procedures rendering the entire process of RFLP analysis free of toxic materials.",
  "language" : "English",
  "identifier_report" : "",
  "publisher_sponsor" : "",
  "publisher_country" : "United Kingdom",
  "source" : "JMT; EDB-88-143436",
  "purl" : "",
  "title" : "Simple salting out procedure for extracting DNA from human nucleated cells",
  "type" : "Journal Article",
  "subject_related" : "",
  "relation" : "Journal Name: Nucleic Acids Res.; (United Kingdom); Journal Volume: 16:3",
  "entry_date" : "2010-12-30",
  "subject_list" : [ "59 BASIC BIOLOGICAL SCIENCES", "DNA", "CYTOLOGICAL TECHNIQUES", "BLOOD CELLS", "CELL NUCLEI", "DEHYDRATION", "HUMAN POPULATIONS", "RFLPS", "SALTING-OUT AGENTS", "SODIUM CHLORIDES", "ALKALI METAL COMPOUNDS", "BIOLOGICAL MATERIALS", "BLOOD", "BODY FLUIDS", "CELL CONSTITUENTS", "CHLORIDES", "CHLORINE COMPOUNDS", "HALIDES", "HALOGEN COMPOUNDS", "MATERIALS", "NUCLEIC ACIDS", "ORGANIC COMPOUNDS", "POPULATIONS", "SODIUM COMPOUNDS", "550200* - Biochemistry" ],
  "publisher_availability" : "",
  "rights" : "",
  "announced_date" : "1988-08-01",
  "type_qualifier" : "",
  "has_fulltext" : false,
  "coverage" : "",
  "identifier" : "",
  "journal_volume" : "16:3",
  "creator" : "Miller, S A; Dykes, D D; Polesky, H F",
  "site_ownership_code" : "JMT",
  "osti_id" : "6998585",
  "journal_issue" : "",
  "resource_type" : "JOUR",
  "format" : "Medium: X; Size: Pages: 1215",
  "journal_name" : [ ],
  "contributing_organizations" : "",
  "citation_location" : "https://www.osti.gov/etdeweb/biblio/6998585",
  "publication_year" : 1988,
  "subject_list_commas" : "59 BASIC BIOLOGICAL SCIENCES, DNA, CYTOLOGICAL TECHNIQUES, BLOOD CELLS, CELL NUCLEI, DEHYDRATION, HUMAN POPULATIONS, RFLPS, SALTING-OUT AGENTS, SODIUM CHLORIDES, ALKALI METAL COMPOUNDS, BIOLOGICAL MATERIALS, BLOOD, BODY FLUIDS, CELL CONSTITUENTS, CHLORIDES, CHLORINE COMPOUNDS, HALIDES, HALOGEN COMPOUNDS, MATERIALS, NUCLEIC ACIDS, ORGANIC COMPOUNDS, POPULATIONS, SODIUM COMPOUNDS, 550200* - Biochemistry",
  "publisher" : "",
  "identifier_other" : "Journal ID: CODEN: NARHA",
  "publisher_research" : "Memorial Blood Center of Minneapolis, MN (USA)",
  "creators_list" : [ "Miller, S A", "Dykes, D D", "Polesky, H F" ],
  "doi" : "https://doi.org/10.1093/nar/16.3.1215"
}