TY - JOUR TI - Cellulolytic properties of an extremely thermophilic anaerobe AB - An extremely thermophilic anaerobe was isolated from a New Zealand hot spring by incubating bacterial mat strands in a medium containing xylan. The Gramreaction-negative organism that was subsequently purified had a temperature optimum of 70deg C and a pH optimum of 7.0. The isolate, designated strain H173, grew on a restricted range of carbon sources. In batch culture H173 could degrade Avicel completely when supplied at 5 or 10 g l{sup -1}. There was an initial growth phase, during which a cellulase complex was produced and carbohydrates fermented to form acetic and lactic acids, followed by a phase where cells were not metabolising but the cellulase complex actively converted cellulose to glucose. When co-cultered with strain Rt8.B1, an ethanologenic extreme thermophile, glucose was fermented to ethanol and acetate, and no reducing sugars accumulated in the medium. In pH controlled batch culture H173 produced an increased amount of lactate and acetate but there was again a phase when reducing sugars accumulated in the medium, and these were converted to ethanol by co-culture with Rt8.B1. (orig.). AU - "Hudson, J A" AU - "Morgan, H W" AU - "Daniel, R M [Waikato Univ., Hamilton (New Zealand). Microbial Biochemistry and Biotechnology Unit]" KW - "09 BIOMASS FUELS" KW - "ENZYMES" KW - "CELLULOLYTIC ACTIVITY" KW - "PRODUCTION" KW - "ANAEROBIC CONDITIONS" KW - "BATCH CULTURE" KW - "BIOSYNTHESIS" KW - "ETHANOL" KW - "THERMOPHILIC CONDITIONS" KW - "XYLANS" KW - "ALCOHOLS" KW - "CARBOHYDRATES" KW - "ENZYME ACTIVITY" KW - "HEMICELLULOSE" KW - "HYDROXY COMPOUNDS" KW - "ORGANIC COMPOUNDS" KW - "POLYSACCHARIDES" KW - "SACCHARIDES" KW - "SYNTHESIS" KW - "090900* - Biomass Fuels- Processing- (1990-)" DO - https://doi.org/10.1007/BF00604939 UR - PB - CY - Germany PY - 1990 DA - 1990-09-01 LA - English J2 - [] VL - 33:6 C1 - C2 - C3 - C4 - C5 - L3 - Journal Name: Applied Microbiology and Biotechnology; (Germany, F.R.); Journal Volume: 33:6 ER -