"TITLE","AUTHORS","SUBJECT","SUBJECT_RELATED","DESCRIPTION","PUBLISHER","AVAILABILITY","RESEARCH_ORG","SPONSORING_ORG","PUBLICATION_COUNTRY","PUBLICATION_DATE","CONTRIBUTING_ORGS","LANGUAGE","RESOURCE_TYPE","TYPE_QUALIFIER","JOURNAL_ISSUE","JOURNAL_VOLUME","RELATION","COVERAGE","FORMAT","IDENTIFIER","REPORT_NUMBER","DOE_CONTRACT_NUMBER","OTHER_IDENTIFIER","DOI","RIGHTS","ENTRY_DATE","OSTI_IDENTIFIER","PURL_URL" "Incomplete separation of radioiodinated thyroid hormones in serum using specific antibodies","Perrild, H; Skovsted, L; Korsgaard Christensen, L [Department of Internal Medicine and Endocrinology, Herlev Hospital, DK-2730 Herlev, Denmark]","62 RADIOLOGY AND NUCLEAR MEDICINE; THYROID HORMONES; SEPARATION PROCESSES; ANTIBODIES; BLOOD SERUM; IODINE 125; LABELLED COMPOUNDS; RADIOIMMUNOASSAY; SPECIFICITY; THYROID; BETA DECAY RADIOISOTOPES; BODY; DAYS LIVING RADIOISOTOPES; ELECTRON CAPTURE RADIOISOTOPES; ENDOCRINE GLANDS; GLANDS; HORMONES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; NUCLEI; ODD-EVEN NUCLEI; ORGANS; PEPTIDE HORMONES; RADIOASSAY; RADIOISOTOPES; TRACER TECHNIQUES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics","","Alkaline Sephadex G-25 columns were used to separate labelled 3,5,3',5'-thyroxine, 3,5,3'-triiodothyronine, 3,3',5'-triiodothyronine and 3,3'-diiodothyronine from the serum binding proteins followed by a quantitative elution of each hormone by coupling to its respective antibody. It is shown that although these antibodies (diluted 1:1500-1:100 000) in our radioimmunoassays are highly specific they show a high degree of non-specific binding when they are used in the concentrations necessary to get a maximal recovery of the hormones in column separating experiments.","","","","","Denmark","1980-01-01","","English","Journal Article","","","95","Journal Name: Acta Endocrinol.; (Denmark); Journal Volume: 95","","Medium: X; Size: Pages: 485-488","","","","Journal ID: CODEN: ACENA","https://doi.org/","","2010-12-30","6443606",""