TY - JOUR TI - Structure and expression of thyroglobulin gene AB - Thyroglobulin is composed of two 300000 dalton polypeptide chains, translated from an 8000 base mRNA. Preparation of a full length cDNA and its cloning in E. coli have lead to the demonstration that the polypeptides of thyroglobulin protomers were identical. Used as molecular probes, the cloned cDNA allowed the isolation of a fragment of thyroglobulin gene. Electron microscopic studies have demonstrated that this gene contains more than 90 % intronic material separating small size exons (<200 bp). Sequencing of bovine thyroglobulin structural gene is in progress. Preliminary results show evidence for the existence of repetitive segments. Availability of cloned DNA complementary to bovine and human thyroglobulin mRNA allows the study of genetic defects of thyroglobulin gene expression in the human and in various animal models. AU - "Vassart, G" AU - "Brocas, H" AU - "Christophe, D" AU - "de Martynoff, G" AU - "Leriche, A" AU - "Mercken, L" AU - "Pohl, V" AU - "van Heuverswyn, B [Institut de Recherche Interdisciplinaire en Biologie Humaine et Nucleaire (IRIBHN), Faculte de Medecine, Universite libre de Bruxelles, Campus Hopital Erasme, Brussels (Belgium)]" KW - "59 BASIC BIOLOGICAL SCIENCES" KW - "THYROGLOBULIN" KW - "BIOSYNTHESIS" KW - "CLONING" KW - "DNA" KW - "GENES" KW - "MESSENGER-RNA" KW - "GLOBULINS" KW - "NUCLEIC ACIDS" KW - "ORGANIC COMPOUNDS" KW - "PROTEINS" KW - "RNA" KW - "SYNTHESIS" KW - "550200* - Biochemistry" KW - "550400 - Genetics" DO - https://doi.org/ UR - PB - CY - France PY - 1982 DA - 1982-01-01 LA - French J2 - [] VL - 43:5 C1 - C2 - C3 - C4 - C5 - L3 - Journal Name: Ann. Endocrinol. (Paris); (France); Journal Volume: 43:5 ER -