{
  "date" : "2013-12-01",
  "identifier_doecontract" : "",
  "subject" : "60 APPLIED LIFE SCIENCES; 36 MATERIALS SCIENCE; CARTILAGE; CELL PROLIFERATION; COLLAGEN; FIBERS; GELATION; HYDROGELS; MECHANICAL PROPERTIES; OLIGOSACCHARIDES",
  "description" : "Articular cartilage has limited repair capability following traumatic injuries and current methods of treatment remain inefficient. Reconstructing cartilage provides a new way for cartilage repair and natural polymers are often used as scaffold because of their biocompatibility and biofunctionality. In this study, we added degummed chopped silk fibers and electrospun silk fibers to the thermosensitive chitosan/glycerophosphate hydrogels to reinforce two hydrogel constructs which were used as scaffold for hyaline cartilage regeneration. The gelation temperature and gelation time of hydrogel were analyzed by the rheometer and vial tilting method. Mechanical characterization was measured by uniaxial compression, indentation and dynamic mechanical analysis assay. Chondrocytes were then harvested from the knee joint of the New Zealand white rabbits and cultured in constructs. The cell proliferation, viability, production of glycosaminoglycans and collagen type II were assessed. The results showed that mechanical properties of the hydrogel were significantly enhanced when a hybrid with two layers of electrospun silk fibers was made. The results of GAG and collagen type II in cell-seeded scaffolds indicate support of the chondrogenic phenotype for chondrocytes with a significant increase in degummed silk fiber–hydrogel composite for GAG content and in two-layer electrospun fiber–hydrogel composite for Col II. It was concluded that these two modified scaffolds could be employed for cartilage tissue engineering. - Highlights: • Chitosan hydrogel composites fabricated by two forms of silk fiber • Silk fibers provide structural support for the hydrogel matrix. • The mechanical properties of hydrogel significantly improved by associating with silk. • Production of GAG and collagen type II was demonstrated within the scaffolds.",
  "language" : "English",
  "identifier_report" : "",
  "publisher_sponsor" : "",
  "publisher_country" : "Netherlands",
  "source" : "NLN",
  "purl" : "",
  "title" : "Enhanced mechanical properties of thermosensitive chitosan hydrogel by silk fibers for cartilage tissue engineering",
  "type" : "Journal Article",
  "subject_related" : "",
  "relation" : "Journal Name: Materials Science and Engineering. C, Biomimetic Materials, Sensors and Systems; Journal Volume: 33; Journal Issue: 8; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)",
  "entry_date" : "2015-06-19",
  "subject_list" : [ "60 APPLIED LIFE SCIENCES", "36 MATERIALS SCIENCE", "CARTILAGE", "CELL PROLIFERATION", "COLLAGEN", "FIBERS", "GELATION", "HYDROGELS", "MECHANICAL PROPERTIES", "OLIGOSACCHARIDES" ],
  "publisher_availability" : "Available from http://dx.doi.org/10.1016/j.msec.2013.07.043",
  "rights" : "",
  "announced_date" : "2015-06-19",
  "type_qualifier" : "",
  "has_fulltext" : false,
  "coverage" : "",
  "identifier" : "PII: S0928-4931(13)00459-1",
  "journal_volume" : "33",
  "creator" : "Mirahmadi, Fereshteh [Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran (Iran, Islamic Republic of)]; National Cell Bank of Iran, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)]; Tafazzoli-Shadpour, Mohammad [Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran (Iran, Islamic Republic of)]; Shokrgozar, Mohammad Ali, E-mail: mashokrgozar@pasteur.ac.ir [National Cell Bank of Iran, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)]; Bonakdar, Shahin [National Cell Bank of Iran, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)]",
  "site_ownership_code" : "NLN",
  "osti_id" : "22344578",
  "journal_issue" : "8",
  "resource_type" : "JOUR",
  "format" : "Medium: X; Size: page(s) 4786-4794",
  "journal_name" : [ ],
  "contributing_organizations" : "",
  "citation_location" : "https://www.osti.gov/etdeweb/biblio/22344578",
  "publication_year" : 2013,
  "subject_list_commas" : "60 APPLIED LIFE SCIENCES, 36 MATERIALS SCIENCE, CARTILAGE, CELL PROLIFERATION, COLLAGEN, FIBERS, GELATION, HYDROGELS, MECHANICAL PROPERTIES, OLIGOSACCHARIDES",
  "publisher" : "",
  "identifier_other" : "Journal ID: ISSN 0928-4931; Other: PII: S0928-4931(13)00459-1; TRN: NL14R9777050912",
  "publisher_research" : "",
  "creators_list" : [ "Mirahmadi, Fereshteh [Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran (Iran, Islamic Republic of)]", "National Cell Bank of Iran, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)]", "Tafazzoli-Shadpour, Mohammad [Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran (Iran, Islamic Republic of)]", "Shokrgozar, Mohammad Ali, E-mail: mashokrgozar@pasteur.ac.ir [National Cell Bank of Iran, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)]", "Bonakdar, Shahin [National Cell Bank of Iran, Pasteur Institute of Iran, Tehran (Iran, Islamic Republic of)]" ],
  "doi" : "https://doi.org/10.1016/J.MSEC.2013.07.043"
}