{
  "date" : "2013-04-03",
  "identifier_doecontract" : "",
  "subject" : "37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; 77 NANOSCIENCE AND NANOTECHNOLOGY; ADSORPTION; AMPEROMETRY; CARBON; DETECTION; FILMS; MAMMARY GLANDS; MOLTEN SALTS; NANOSTRUCTURES; NEOPLASMS; SENSITIVITY; STABILITY; SURFACE AREA; SURFACES; TOLUIDINE BLUE",
  "description" : "Highlights: ► The immunosensor is designed to determine breast cancer susceptibility gene. ► Mesoporous carbon nanospheres (MCN) have great adsorption capacity. ► MCN could enhance the electroactivity of toluidine blue. ► Room temperature ionic liquid should increase the electrochemical signal. -- Abstract: In this contribution, mesoporous carbon nanospheres (MCN) were used to fabricate a label-free electrochemical immunosensor for breast cancer susceptibility gene (BRCAl). The detection platform was constructed by conjugation of anti-BRCA1 on glassy carbon electrodes which were modified by mesoporous carbon nanospheres–toluidine blue nanocomposite (MCN–TB)/room temperature ionic-liquid (RTIL) composited film. TB was adsorbed onto MCN and acted as a redox probe. The electroactivity of TB was greatly enhanced in the presence of MCN. The good conductivity of MCN and BMIM·BF{sub 4} could promote the electron transfer and thus enhance the detection sensitivity. Moreover, the large surface area of MCN and the protein-binding properties of BMIM·BF{sub 4} could greatly increase the antibody loading. The specific antibody–antigen immunoreaction on the electrode surface resulted in a decrease of amperometric signal of the electrode. Under optimized conditions, the amperometric signal decreased linearly with BRCAl concentration in the range of 0.01–15 ng mL{sup −1} with a low detection limit of 3.97 pg mL{sup −1}. The immunosensor exhibits high sensitivity, good selectivity and stability.",
  "language" : "English",
  "identifier_report" : "",
  "publisher_sponsor" : "",
  "publisher_country" : "Netherlands",
  "source" : "NLN",
  "purl" : "",
  "title" : "Construction of label-free electrochemical immunosensor on mesoporous carbon nanospheres for breast cancer susceptibility gene",
  "type" : "Journal Article",
  "subject_related" : "",
  "relation" : "Journal Name: Analytica Chimica Acta; Journal Volume: 770; Other Information: Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)",
  "entry_date" : "2014-04-28",
  "subject_list" : [ "37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY", "77 NANOSCIENCE AND NANOTECHNOLOGY", "ADSORPTION", "AMPEROMETRY", "CARBON", "DETECTION", "FILMS", "MAMMARY GLANDS", "MOLTEN SALTS", "NANOSTRUCTURES", "NEOPLASMS", "SENSITIVITY", "STABILITY", "SURFACE AREA", "SURFACES", "TOLUIDINE BLUE" ],
  "publisher_availability" : "Available from http://dx.doi.org/10.1016/j.aca.2013.01.066",
  "rights" : "",
  "announced_date" : "2014-04-28",
  "type_qualifier" : "",
  "has_fulltext" : false,
  "coverage" : "",
  "identifier" : "PII: S0003-2670(13)00215-8",
  "journal_volume" : "770",
  "creator" : "Fan, Haixia; Zhang, Yong; Wu, Dan; Ma, Hongmin; Li, Xiaojing; Li, Yan; Wang, Huan; Li, He; Du, Bin [Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (China)]; Wei, Qin [Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (China)]",
  "site_ownership_code" : "NLN",
  "osti_id" : "22219704",
  "journal_issue" : "",
  "resource_type" : "JOUR",
  "format" : "Medium: X; Size: page(s) 62-67",
  "journal_name" : [ ],
  "contributing_organizations" : "",
  "citation_location" : "https://www.osti.gov/etdeweb/biblio/22219704",
  "publication_year" : 2013,
  "subject_list_commas" : "37 INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY, 77 NANOSCIENCE AND NANOTECHNOLOGY, ADSORPTION, AMPEROMETRY, CARBON, DETECTION, FILMS, MAMMARY GLANDS, MOLTEN SALTS, NANOSTRUCTURES, NEOPLASMS, SENSITIVITY, STABILITY, SURFACE AREA, SURFACES, TOLUIDINE BLUE",
  "publisher" : "",
  "identifier_other" : "Journal ID: ISSN 0003-2670; CODEN: ACACAM; Other: PII: S0003-2670(13)00215-8; TRN: NL14R0017040659",
  "publisher_research" : "",
  "creators_list" : [ "Fan, Haixia", "Zhang, Yong", "Wu, Dan", "Ma, Hongmin", "Li, Xiaojing", "Li, Yan", "Wang, Huan", "Li, He", "Du, Bin [Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (China)]", "Wei, Qin [Key Laboratory of Chemical Sensing and Analysis in Universities of Shandong, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022 (China)]" ],
  "doi" : "https://doi.org/10.1016/J.ACA.2013.01.066"
}