TY - JOUR TI - Respiratory syncytial virus M2-1 protein induces the activation of nuclear factor kappa B AB - Respiratory syncytial virus (RSV) induces the production of a number of cytokines and chemokines by activation of nuclear factor kappa B (NF-{kappa}B). The activation of NF-{kappa}B has been shown to depend on viral replication in the infected cells. In this study, we demonstrate that expression of RSV M2-1 protein, a transcriptional processivity and anti-termination factor, is sufficient to activate NF-{kappa}B in A549 cells. Electromobility shift assays show increased NF-{kappa}B complexes in the nuclei of M2-1-expressing cells. M2-1 protein is found in nuclei of M2-1-expressing cells and in RSV-infected cells. Co-immunoprecipitations of nuclear extracts of M2-1-expressing cells and of RSV-infected cells revealed an association of M2-1 with Rel A protein. Furthermore, the activation of NF-{kappa}B depends on the C-terminus of the RSV M2-1 protein, as shown by NF-{kappa}B-induced gene expression of a reporter gene construct. AU - "Reimers, Kerstin [Klinik fuer Plastische, Hand-und Wiederherstellungschirurgie, Podbielskistrasse 380, D-30659 Hannover (Germany)]" AU - "Buchholz, Katja [Institut fuer Medizinische Mikrobiologie, Otto-von-Guericke-Universitaet Magdeburg, Leipzigerstrasse 44, D-39120 Magdeburg (Germany)]" AU - "Werchau, Hermann [Institut fuer Medizinische Mikrobiologie, Otto-von-Guericke-Universitaet Magdeburg, Leipzigerstrasse 44, D-39120 Magdeburg (Germany)]" KW - "60 APPLIED LIFE SCIENCES" KW - "CELL NUCLEI" KW - "GENES" KW - "INFECTIOUS DISEASES" KW - "LYMPHOKINES" KW - "RESPIRATORY SYSTEM DISEASES" KW - "VIRUSES" DO - https://doi.org/10.1016/j.virol.2004.10.031 UR - PB - CY - United States PY - 2005 DA - 2005-01-20 LA - English J2 - [] VL - 331 C1 - C2 - C3 - C4 - C5 - L3 - Journal Name: Virology; Journal Volume: 331; Journal Issue: 2; Other Information: DOI: 10.1016/j.virol.2004.10.031; PII: S0042-6822(04)00682-8; Copyright (c) 2004 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved; Country of input: International Atomic Energy Agency (IAEA) ER -