Oxygen-resistant hydrogenases and methods for designing and making same
Abstract
The invention provides oxygen- resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.
- Inventors:
-
- Golden, CO
- Lakewood, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 986476
- Patent Number(s):
- 7501270
- Application Number:
- 10/553,097
- Assignee:
- Allaince for Sustainable Energy, LLC (Golden, CO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-99GO10337
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
King, Paul, Ghirardi, Maria L, and Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same. United States: N. p., 2009.
Web.
King, Paul, Ghirardi, Maria L, & Seibert, Michael. Oxygen-resistant hydrogenases and methods for designing and making same. United States.
King, Paul, Ghirardi, Maria L, and Seibert, Michael. Tue .
"Oxygen-resistant hydrogenases and methods for designing and making same". United States. https://www.osti.gov/servlets/purl/986476.
@article{osti_986476,
title = {Oxygen-resistant hydrogenases and methods for designing and making same},
author = {King, Paul and Ghirardi, Maria L and Seibert, Michael},
abstractNote = {The invention provides oxygen- resistant iron-hydrogenases ([Fe]-hydrogenases) for use in the production of H2. Methods used in the design and engineering of the oxygen-resistant [Fe]-hydrogenases are disclosed, as are the methods of transforming and culturing appropriate host cells with the oxygen-resistant [Fe]-hydrogenases. Finally, the invention provides methods for utilizing the transformed, oxygen insensitive, host cells in the bulk production of H.sub.2 in a light catalyzed reaction having water as the reactant.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 10 00:00:00 EDT 2009},
month = {Tue Mar 10 00:00:00 EDT 2009}
}
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