Title: Methods for improved selectivity in photo-activation and detection of molecular diagnostic agents

Abstract

A method for the imaging of a particular volume of plant or animal tissue, wherein the plant or animal tissue contains at least one photo-active molecular agent. The method comprises the steps of treating the particular volume of the plant or animal tissue with light sufficient to promote a simultaneous two-photon excitation of the photo-active molecular agent contained in the particular volume of the plant or animal tissue, photo-activating at least one of the at least one photo-active molecular agent in the particular volume of the plant or animal tissue, thereby producing at least one photo-activated molecular agent, wherein the at least one photo-activated molecular agent emits energy, detecting the energy emitted by the at least one photo-activated molecular agent, and producing a detected energy signal which is characteristic of the particular volume of plant or animal tissue. The present invention also provides a method for the imaging of a particular volume of material, wherein the material contains at least one photo-active molecular agent.

Inventors:

Wachter, Eric A ^[1]; Fisher, Walter G ^[2]; Dees, H Craig ^[2]

---

+ Show Inventor Affiliations

1. Oak Ridge, TN
2. Knoxville, TN

Issue Date:

Tue Mar 18 00:00:00 EDT 2008

Research Org.:

Lockheed Martin Energy Systems, Inc.

Sponsoring Org.:

USDOE

OSTI Identifier:

970385

Patent Number(s):

7346387

Application Number:

09/072,963

Assignee:

Provectus Devicetech, Inc. (Knoxville, TN)

Patent Classifications (CPCs):

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

Show more

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS

A61B2017/00057 - {Light}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

A61K41/008 - {Two-Photon or Multi-Photon PDT, e.g. with upconverting dyes or photosensitisers}
A61K49/0017 - {Fluorescence in vivo}
A61K49/0039 - {Coumarin dyes}
A61K49/0043 - {Fluorescein, used in vivo}

A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61N - ELECTROTHERAPY

A61N5/062 - {Photodynamic therapy, i.e. excitation of an agent}

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors

Show less

DOE Contract Number:  

AC05-84OR21400

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

60 APPLIED LIFE SCIENCES