High throughput protein production screening
Abstract
Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.
- Inventors:
-
- Walnut Creek, CA
- Oakland, CA
- San Ramon, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 968305
- Patent Number(s):
- 7585815
- Application Number:
- 10/884,783
- Assignee:
- Lawrence Livermore National Security, LLC (Livermore, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Beernink, Peter T, Coleman, Matthew A, and Segelke, Brent W. High throughput protein production screening. United States: N. p., 2009.
Web.
Beernink, Peter T, Coleman, Matthew A, & Segelke, Brent W. High throughput protein production screening. United States.
Beernink, Peter T, Coleman, Matthew A, and Segelke, Brent W. Tue .
"High throughput protein production screening". United States. https://www.osti.gov/servlets/purl/968305.
@article{osti_968305,
title = {High throughput protein production screening},
author = {Beernink, Peter T and Coleman, Matthew A and Segelke, Brent W},
abstractNote = {Methods, compositions, and kits for the cell-free production and analysis of proteins are provided. The invention allows for the production of proteins from prokaryotic sequences or eukaryotic sequences, including human cDNAs using PCR and IVT methods and detecting the proteins through fluorescence or immunoblot techniques. This invention can be used to identify optimized PCR and WT conditions, codon usages and mutations. The methods are readily automated and can be used for high throughput analysis of protein expression levels, interactions, and functional states.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 08 00:00:00 EDT 2009},
month = {Tue Sep 08 00:00:00 EDT 2009}
}
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