Fluid delivery system for capillary electrophoretic applications
Abstract
An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.
- Inventors:
- Issue Date:
- Research Org.:
- Spectrumedix Corporation, State College, PA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 879604
- Patent Number(s):
- 6375819
- Application Number:
- 09/373,909
- Assignee:
- Spectrumedix Corporation (State College, PA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG02-97ER82461
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 1999 Aug 13
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, and Sharer, Michael V. Fluid delivery system for capillary electrophoretic applications. United States: N. p., 2002.
Web.
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, & Sharer, Michael V. Fluid delivery system for capillary electrophoretic applications. United States.
Li, Qingbo, Liu, Changsheng, Kane, Thomas E., Kernan, John R., Sonnenschein, Bernard, and Sharer, Michael V. Tue .
"Fluid delivery system for capillary electrophoretic applications". United States. https://www.osti.gov/servlets/purl/879604.
@article{osti_879604,
title = {Fluid delivery system for capillary electrophoretic applications},
author = {Li, Qingbo and Liu, Changsheng and Kane, Thomas E. and Kernan, John R. and Sonnenschein, Bernard and Sharer, Michael V.},
abstractNote = {An automated electrophoretic system is disclosed. The system employs a capillary cartridge having a plurality of capillary tubes. The cartridge has a first array of capillary ends projecting from one side of a plate. The first array of capillary ends are spaced apart in substantially the same manner as the wells of a microtitre tray of standard size. This allows one to simultaneously perform capillary electrophoresis on samples present in each of the wells of the tray. The system includes a stacked, dual carrousel arrangement to eliminate cross-contamination resulting from reuse of the same buffer tray on consecutive executions from electrophoresis. The system also has a gel delivery module containing a gel syringe/a stepper motor or a high pressure chamber with a pump to quickly and uniformly deliver gel through the capillary tubes. The system further includes a multi-wavelength beam generator to generate a laser beam which produces a beam with a wide range of wavelengths. An off-line capillary reconditioner thoroughly cleans a capillary cartridge to enable simultaneous execution of electrophoresis with another capillary cartridge. The streamlined nature of the off-line capillary reconditioner offers the advantage of increased system throughput with a minimal increase in system cost.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 23 00:00:00 EDT 2002},
month = {Tue Apr 23 00:00:00 EDT 2002}
}