Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics
Abstract
PCR (polymerase chain reaction) primers for the detection of certain Bacillus species, such as Bacillus anthracis. The primers specifically amplify only DNA found in the target species and can distinguish closely related species. Species-specific PCR primers for Bacillus anthracis, Bacillus globigii and Clostridium perfringens are disclosed. The primers are directed to unique sequences within sasp (small acid soluble protein) genes.
- Inventors:
-
- Decatur, GA
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- OSTI Identifier:
- 874841
- Patent Number(s):
- 6472155
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- AC03-76SF00098
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- species; specific; identification; spore-producing; microbes; sequence; acid-soluble; spore; coat; proteins; amplification; based; diagnostics; pcr; polymerase; chain; reaction; primers; detection; bacillus; anthracis; specifically; amplify; dna; found; target; distinguish; closely; related; species-specific; globigii; clostridium; perfringens; disclosed; directed; unique; sequences; sasp; acid; soluble; protein; genes; chain reaction; /435/999/
Citation Formats
McKinney, Nancy. Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics. United States: N. p., 2002.
Web.
McKinney, Nancy. Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics. United States.
McKinney, Nancy. Tue .
"Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics". United States. https://www.osti.gov/servlets/purl/874841.
@article{osti_874841,
title = {Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics},
author = {McKinney, Nancy},
abstractNote = {PCR (polymerase chain reaction) primers for the detection of certain Bacillus species, such as Bacillus anthracis. The primers specifically amplify only DNA found in the target species and can distinguish closely related species. Species-specific PCR primers for Bacillus anthracis, Bacillus globigii and Clostridium perfringens are disclosed. The primers are directed to unique sequences within sasp (small acid soluble protein) genes.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
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Works referenced in this record:
Cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus".
journal, January 1987
- Sun, D. X.; Setlow, P.
- Journal of Bacteriology, Vol. 169, Issue 7
Cloning and nucleotide sequence of three genes coding for small, acid-soluble proteins of Clostridium perfringens spores
journal, January 1991
- Cabrera-Martinez, Rosa Martha; Setlow, Peter
- FEMS Microbiology Letters, Vol. 77, Issue 2-3