DOE Patents DOE Patents
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search DOE Patents

Title: Use of continuous/contiguous stacking hybridization as a diagnostic tool

Abstract

A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. A method is also provided for determining the length of a repeat sequence in DNA or RNA, and also for determining the base sequence of unknown DNA or RNA.

Inventors:
 [1];  [1];  [1];  [1];  [2]
+ Show Inventor Affiliations
  1. Moscow, RU
  2. Kiev, RU
Issue Date:
Research Org.:
Argonne National Laboratory (ANL), Argonne, IL (United States)
OSTI Identifier:
874683
Patent Number(s):
6440671
Assignee:
The University of Chicago (Chicago, IL)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
Show more
DOE Contract Number:  
W-31109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
continuouscontiguous; stacking; hybridization; diagnostic; tool; method; detecting; disease-associated; alleles; patient; genetic; material; provided; oligonucleotide; molecules; synthesized; compliment; base; sequences; disease; associated; immobilized; predetermined; position; substrate; contacted; form; duplexes; extend; length; tagged; incorporate; universal; bases; mixture; guanine; cytosine; thymine; adenine; complementary; nucleotide; strands; fluorochrome; radiates; light; wavelength; treated; washed; patterns; radiating; compared; diseases; prepared; identical; substrates; determining; repeat; sequence; dna; rna; base sequence; /435/436/999/

Citation Formats

Mirzabekov, Andrei Darievich, Kirillov, Eugene Vladislavovich, Parinov, Sergei Valeryevich, Barski, Victor Evgenievich, and Dubiley, Svetlana Alekseevna. Use of continuous/contiguous stacking hybridization as a diagnostic tool. United States: N. p., 2002. Web.
Copy to clipboard
Mirzabekov, Andrei Darievich, Kirillov, Eugene Vladislavovich, Parinov, Sergei Valeryevich, Barski, Victor Evgenievich, & Dubiley, Svetlana Alekseevna. Use of continuous/contiguous stacking hybridization as a diagnostic tool. United States.
Copy to clipboard
Mirzabekov, Andrei Darievich, Kirillov, Eugene Vladislavovich, Parinov, Sergei Valeryevich, Barski, Victor Evgenievich, and Dubiley, Svetlana Alekseevna. Tue . "Use of continuous/contiguous stacking hybridization as a diagnostic tool". United States. https://www.osti.gov/servlets/purl/874683.
Copy to clipboard
@article{osti_874683,
title = {Use of continuous/contiguous stacking hybridization as a diagnostic tool},
author = {Mirzabekov, Andrei Darievich and Kirillov, Eugene Vladislavovich and Parinov, Sergei Valeryevich and Barski, Victor Evgenievich and Dubiley, Svetlana Alekseevna},
abstractNote = {A method for detecting disease-associated alleles in patient genetic material is provided whereby a first group of oligonucleotide molecules, synthesized to compliment base sequences of the disease associated alleles is immobilized on a predetermined position on a substrate, and then contacted with patient genetic material to form duplexes. The duplexes are then contacted with a second group of oligonucleotide molecules which are synthesized to extend the predetermined length of the oligonucleotide molecules of the first group, and where each of the oligonucleotide molecules of the second group are tagged and either incorporate universal bases or a mixture of guanine, cytosine, thymine, and adenine, or complementary nucleotide strands that are tagged with a different fluorochrome which radiates light at a predetermined wavelength. The treated substrate is then washed and the light patterns radiating therefrom are compared with predetermined light patterns of various diseases that were prepared on identical substrates. A method is also provided for determining the length of a repeat sequence in DNA or RNA, and also for determining the base sequence of unknown DNA or RNA.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
Copy to clipboard
Patent:
View Patent
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

A method for DNA sequencing by hybridization with oligonucleotide matrix
journal, January 1991

    [ × clear filter / sort ]

    Similar Records in DOE Patents and OSTI.GOV collections: