Title: Organo luminescent semiconductor nanocrystal probes for biological applications and process for making and using such probes

Abstract

A semiconductor nanocrystal compound is described capable of linking to an affinity molecule. The compound comprises (1) a semiconductor nanocrystal capable of emitting electromagnetic radiation and/or absorbing energy, and/or scattering or diffracting electromagnetic radiation--when excited by an electromagnetic radiation source or a particle beam; and (2) at least one linking agent, having a first portion linked to the semiconductor nanocrystal and a second portion capable of linking to an affity molecule. The compound is linked to an affinity molecule to form a semiconductor nanocrystal probe capable of bonding with a detectable substance. Subsequent exposure to excitation energy will excite the semiconductor nanocrystal in he probe, causing the emission of electromagnetic radiation. Further described are processes for respectively: making the semiconductor nanocrystal compound; making the semiconductor nanocrystal probe; and using the probe to determine the presence of a detectable substance in a material.

Inventors:

Weiss, Shimon ^[1]; Bruchez, Jr., Marcel ^[2]; Alivisatos, Paul ^[3]

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+ Show Inventor Affiliations

1. Pinole, CA
2. (Albany, CA)
3. Oakland, CA

Issue Date:

Tue Jan 01 00:00:00 EST 2002

Research Org.:

Univ. of California (United States)

OSTI Identifier:

874612

Patent Number(s):

6423551

Assignee:

The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

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B - PERFORMING OPERATIONS B82 - NANOTECHNOLOGY B82Y - SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES

B82Y15/00 - Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6816 - characterised by the detection means
C12Q2563/107 - fluorescence

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/533 - with fluorescent label
G01N33/542 - with steric inhibition or signal modification, e.g. fluorescent quenching
G01N33/54373 - {involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings}
G01N33/588 - {with semiconductor nanocrystal label, e.g. quantum dots}

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T428/2982 - Particulate matter [e.g., sphere, flake, etc.]
Y10T428/2989 - Microcapsule with solid core [includes liposome]
Y10T428/2991 - Coated
Y10T428/2993 - Silicic or refractory material containing [e.g., tungsten oxide, glass, cement, etc.]
Y10T428/2995 - Silane, siloxane or silicone coating
Y10T428/2996 - Glass particles or spheres
Y10T436/24 - Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

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DOE Contract Number:  

AC03-76SF00098

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

organo; luminescent; semiconductor; nanocrystal; probes; biological; applications; process; compound; described; capable; linking; affinity; molecule; emitting; electromagnetic; radiation; andor; absorbing; energy; scattering; diffracting; radiation-when; excited; source; particle; beam; agent; portion; linked; affity; form; probe; bonding; detectable; substance; subsequent; exposure; excitation; excite; causing; emission; processes; determine; presence; material; electromagnetic radiation; particle beam; radiation source; semiconductor nanocrystal; /436/424/428/