Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches
Abstract
Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.
- Inventors:
-
- Pleasanton, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 874322
- Patent Number(s):
- 6365355
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- chimeric; proteins; detection; quantitation; dna; mutations; sequence; variations; damage; mismatches; mutation; binding; activity; nuclease; synthesized; recombinant; technology; formula; a-l-b; b-l-a; peptide; linker; useful; identification; including; cutting; detected; dna sequence; nuclease activity; /435/530/999/
Citation Formats
McCutchen-Maloney, Sandra L. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches. United States: N. p., 2002.
Web.
McCutchen-Maloney, Sandra L. Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches. United States.
McCutchen-Maloney, Sandra L. Tue .
"Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches". United States. https://www.osti.gov/servlets/purl/874322.
@article{osti_874322,
title = {Chimeric proteins for detection and quantitation of DNA mutations, DNA sequence variations, DNA damage and DNA mismatches},
author = {McCutchen-Maloney, Sandra L},
abstractNote = {Chimeric proteins having both DNA mutation binding activity and nuclease activity are synthesized by recombinant technology. The proteins are of the general formula A-L-B and B-L-A where A is a peptide having DNA mutation binding activity, L is a linker and B is a peptide having nuclease activity. The chimeric proteins are useful for detection and identification of DNA sequence variations including DNA mutations (including DNA damage and mismatches) by binding to the DNA mutation and cutting the DNA once the DNA mutation is detected.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 2002},
month = {Tue Jan 01 00:00:00 EST 2002}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Mutation detection using immobilized mismatch binding protein (MutS)
journal, January 1995
- Wagner, Robert; Debble, Paul; Radman, Miroslav
- Nucleic Acids Research, Vol. 23, Issue 19
[37] Affinity selection of polymerase chain reaction products by DNA-binding proteins
book, January 1993
- Lew, Andrew M.; Marshall, Vikki M.; Kemp, David J.
- Methods in Enzymology
CASTing for multicomponent DNA-binding complexes
journal, March 1993
- Wright, Woodring E.; Funk, Walter D.
- Trends in Biochemical Sciences, Vol. 18, Issue 3