Microbial methods of reducing technetium
Abstract
The present invention is directed toward a method for microbial reduction of a technetium compound to form other compounds of value in medical imaging. The technetium compound is combined in a mixture with non-growing microbial cells which contain a technetium-reducing enzyme system, a stabilizing agent and an electron donor in a saline solution under anaerobic conditions. The mixture is substantially free of an inorganic technetium reducing agent and its reduction products. The resulting product is Tc of lower oxidation states, the form of which can be partially controlled by the stabilizing agent. It has been discovered that the microorganisms Shewanella alga, strain Bry and Shewanelia putrifacians, strain CN-32 contain the necessary enzyme systems for technetium reduction and can form both mono nuclear and polynuclear reduced Tc species depending on the stabilizing agent.
- Inventors:
-
- Richland, WA
- Greybull, WY
- Benton City, WA
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 873672
- Patent Number(s):
- 6218171
- Application Number:
- 09/398,834
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
- DOE Contract Number:
- AC06-76RL01830
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- microbial; methods; reducing; technetium; directed; method; reduction; compound; form; compounds; value; medical; imaging; combined; mixture; non-growing; cells; contain; technetium-reducing; enzyme; stabilizing; agent; electron; donor; saline; solution; anaerobic; conditions; substantially; free; inorganic; products; resulting; product; tc; oxidation; partially; controlled; discovered; microorganisms; shewanella; alga; strain; bry; shewanelia; putrifacians; cn-32; systems; mono; nuclear; polynuclear; reduced; species; depending; saline solution; resulting product; substantially free; reducing agent; anaerobic conditions; electron donor; stabilizing agent; microbial reduction; medical imaging; technetium compound; microbial cell; aerobic conditions; /435/999/
Citation Formats
Wildung, Raymond E, Garland, Thomas R, Gorby, Yuri A, Hess, Nancy J, Li, Shu-Mei W, and Plymale, Andrew E. Microbial methods of reducing technetium. United States: N. p., 2001.
Web.
Wildung, Raymond E, Garland, Thomas R, Gorby, Yuri A, Hess, Nancy J, Li, Shu-Mei W, & Plymale, Andrew E. Microbial methods of reducing technetium. United States.
Wildung, Raymond E, Garland, Thomas R, Gorby, Yuri A, Hess, Nancy J, Li, Shu-Mei W, and Plymale, Andrew E. Mon .
"Microbial methods of reducing technetium". United States. https://www.osti.gov/servlets/purl/873672.
@article{osti_873672,
title = {Microbial methods of reducing technetium},
author = {Wildung, Raymond E and Garland, Thomas R and Gorby, Yuri A and Hess, Nancy J and Li, Shu-Mei W and Plymale, Andrew E},
abstractNote = {The present invention is directed toward a method for microbial reduction of a technetium compound to form other compounds of value in medical imaging. The technetium compound is combined in a mixture with non-growing microbial cells which contain a technetium-reducing enzyme system, a stabilizing agent and an electron donor in a saline solution under anaerobic conditions. The mixture is substantially free of an inorganic technetium reducing agent and its reduction products. The resulting product is Tc of lower oxidation states, the form of which can be partially controlled by the stabilizing agent. It has been discovered that the microorganisms Shewanella alga, strain Bry and Shewanelia putrifacians, strain CN-32 contain the necessary enzyme systems for technetium reduction and can form both mono nuclear and polynuclear reduced Tc species depending on the stabilizing agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Mon Jan 01 00:00:00 EST 2001},
month = {Mon Jan 01 00:00:00 EST 2001}
}
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Works referenced in this record:
The biomedical chemistry of technetium and rhenium
journal, January 1998
- R. Dilworth, Jonathan; J. Parrott, Suzanne
- Chemical Society Reviews, Vol. 27, Issue 1
Technetium-99m labelling of bis-oxime ligands
journal, May 1994
- Jackson, Graham E.; Byrne, Michael J.; Fakier, Hashiem
- Applied Radiation and Isotopes, Vol. 45, Issue 5
Technetium reduction and precipitation by sulfate‐reducing bacteria
journal, January 1998
- Lloyd, J. R.; Nolting, H. ‐F.; Solé, V. A.
- Geomicrobiology Journal, Vol. 15, Issue 1
Technetium Sources and Behavior in the Environment1
journal, January 1979
- Wildung, R. E.; McFadden, K. M.; Garland, T. R.
- Journal of Environment Quality, Vol. 8, Issue 2
Dissimilatory Metal Reduction
journal, October 1993
- Lovley, Derek R.
- Annual Review of Microbiology, Vol. 47, Issue 1
Methods of Preparation of Some Carrier-Free Radioisotopes Involving Sorption on Alumina
report, May 1958
- Tucker, W.; Greene, M.; Weiss, A.
Bioaccumulation and Chemical Modification of Tc by Soil Bacteria
journal, January 1989
- Henrot, Jacqueline
- Health Physics, Vol. 57, Issue 2