Compositions and methods for detecting gene rearrangements and translocations
Abstract
Disclosed is a series of nucleic acid probes for use in diagnosing and monitoring certain types of leukemia using, e.g., Southern and Northern blot analyses and fluorescence in situ hybridization (FISH). These probes detect rearrangements, such as translocations involving chromosome band 11q23 with other chromosomes bands, including 4q21, 6q27, 9p22, 19p13.3, in both dividing leukemic cells and interphase nuclei. The breakpoints in all such translocations are clustered within an 8.3 kb BamHI genomic region of the MLL gene. A novel 0.7 kb BamH1 cDNA fragment derived from this gene detects rearrangements on Southern blot analysis with a single BamHI restriction digest in all patients with the common 11q23 translocations and in patients with other 11q23 anomalies. Northern blot analyses are presented demonstrating that the MLL gene has multiple transcripts and that transcript size differentiates leukemic cells from normal cells. Also disclosed are MLL fusion proteins, MLL protein domains and anti-MLL antibodies.
- Inventors:
-
- Chicago, IL
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 873254
- Patent Number(s):
- 6121419
- Assignee:
- Arch Development Corp. (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS
- DOE Contract Number:
- FG02-86ER60408
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- compositions; methods; detecting; rearrangements; translocations; disclosed; series; nucleic; acid; probes; diagnosing; monitoring; types; leukemia; southern; northern; blot; analyses; fluorescence; situ; hybridization; fish; detect; involving; chromosome; band; 11q23; chromosomes; bands; including; 4q21; 6q27; 9p22; 19p13; dividing; leukemic; cells; interphase; nuclei; breakpoints; clustered; kb; bamhi; genomic; region; mll; novel; bamh1; cdna; fragment; derived; detects; analysis; single; restriction; digest; patients; common; anomalies; demonstrating; multiple; transcripts; transcript; size; differentiates; normal; fusion; proteins; protein; domains; anti-mll; antibodies; normal cells; acid probes; nucleic acid; acid probe; situ hybridization; dna fragment; blot analyses; fusion protein; fusion proteins; interphase nuclei; probes detect; /530/
Citation Formats
Rowley, Janet D, and Diaz, Manuel O. Compositions and methods for detecting gene rearrangements and translocations. United States: N. p., 2000.
Web.
Rowley, Janet D, & Diaz, Manuel O. Compositions and methods for detecting gene rearrangements and translocations. United States.
Rowley, Janet D, and Diaz, Manuel O. Sat .
"Compositions and methods for detecting gene rearrangements and translocations". United States. https://www.osti.gov/servlets/purl/873254.
@article{osti_873254,
title = {Compositions and methods for detecting gene rearrangements and translocations},
author = {Rowley, Janet D and Diaz, Manuel O},
abstractNote = {Disclosed is a series of nucleic acid probes for use in diagnosing and monitoring certain types of leukemia using, e.g., Southern and Northern blot analyses and fluorescence in situ hybridization (FISH). These probes detect rearrangements, such as translocations involving chromosome band 11q23 with other chromosomes bands, including 4q21, 6q27, 9p22, 19p13.3, in both dividing leukemic cells and interphase nuclei. The breakpoints in all such translocations are clustered within an 8.3 kb BamHI genomic region of the MLL gene. A novel 0.7 kb BamH1 cDNA fragment derived from this gene detects rearrangements on Southern blot analysis with a single BamHI restriction digest in all patients with the common 11q23 translocations and in patients with other 11q23 anomalies. Northern blot analyses are presented demonstrating that the MLL gene has multiple transcripts and that transcript size differentiates leukemic cells from normal cells. Also disclosed are MLL fusion proteins, MLL protein domains and anti-MLL antibodies.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
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