Streptavidin mutants
Abstract
The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.
- Inventors:
-
- 1 Longfellow Pl. #2715, Boston, MA 02114
- 11 Bay State Rd. #6, Boston, MA 02215
- 8 Hillcrest Rd., Medfield, MA 02050
- 270 Babcock St., #7F, Boston, MA 02215
- 9605 Genesee Ave., #E2, San Diego, CA 92121
- Issue Date:
- Research Org.:
- Boston University
- OSTI Identifier:
- 872848
- Patent Number(s):
- 6022951
- Assignee:
- Sano, Takeshi (1 Longfellow Pl. #2715, Boston, MA 02114);Cantor, Charles R. (11 Bay State Rd. #6, Boston, MA 02215);Vajda, Sandor (8 Hillcrest Rd., Medfield, MA 02050);Reznik, Gabriel O. (270 Babcock St., #7F, Boston, MA 02215);Smith, Cassandra L. (11 Bay State Rd. #6, Boston, MA 02215);Pandori, Mark W. (9605 Genesee Ave., #E2, San Diego, CA 92121)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FG02-93ER61656
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- streptavidin; mutants; relates; proteins; peptides; altered; physical; properties; increased; stability; decreased; affinity; binding; biotin; methods; detection; identification; separation; isolation; targets; reduced; allows; streptavidin-biotin; coupling; systems; remove; partners; useful; purification; functional; viable; cells; nucleic; acids; encode; recombinant; bacteria; yeast; mammalian; contain; binding partner; binding partners; physical properties; nucleic acid; nucleic acids; mammalian cells; reduced affinity; recombinant cells; viable cells; isolation systems; altered physical; increased stability; functional proteins; streptavidin protein; /530/
Citation Formats
Sano, Takeshi, Cantor, Charles R, Vajda, Sandor, Reznik, Gabriel O, Smith, Cassandra L, and Pandori, Mark W. Streptavidin mutants. United States: N. p., 2000.
Web.
Sano, Takeshi, Cantor, Charles R, Vajda, Sandor, Reznik, Gabriel O, Smith, Cassandra L, & Pandori, Mark W. Streptavidin mutants. United States.
Sano, Takeshi, Cantor, Charles R, Vajda, Sandor, Reznik, Gabriel O, Smith, Cassandra L, and Pandori, Mark W. Sat .
"Streptavidin mutants". United States. https://www.osti.gov/servlets/purl/872848.
@article{osti_872848,
title = {Streptavidin mutants},
author = {Sano, Takeshi and Cantor, Charles R and Vajda, Sandor and Reznik, Gabriel O and Smith, Cassandra L and Pandori, Mark W},
abstractNote = {The present invention relates to streptavidin proteins and peptides having a altered physical properties such as an increased stability or increased or decreased affinity for binding biotin. The invention also relates to methods for the detection, identification, separation and isolation of targets using streptavidin proteins or peptides. Streptavidin with increased or reduced affinity allows for the use of the streptavidin-biotin coupling systems for detection and isolation systems wherein it is necessary to remove of one or the other of the binding partners. Such systems are useful for the purification of functional proteins and viable cells. The invention also relates to nucleic acids which encode these streptavidin proteins and peptides and to recombinant cells such as bacteria, yeast and mammalian cells which contain these nucleic acids.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 2000},
month = {Sat Jan 01 00:00:00 EST 2000}
}
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