Methods for immobilizing nucleic acids on a gel substrate
Abstract
A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.
- Inventors:
-
- Moscow, RU
- Samara, RU
- Issue Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- OSTI Identifier:
- 872654
- Patent Number(s):
- 5981734
- Assignee:
- University of Chicago (Chicago, IL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07H - SUGARS
C - CHEMISTRY C08 - ORGANIC MACROMOLECULAR COMPOUNDS C08F - MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- DOE Contract Number:
- W-31109-ENG-38
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- methods; immobilizing; nucleic; acids; substrate; method; labeling; oligonucleotide; molecules; dna; provided; comprising; modifying; create; chemically; active; contacting; activated; fluorescent; dyes; region; preparing; immobilization; contain; desired; functional; covalently; interact; moieties; biomolecules; copolymerization; allyl-substituted; oligonucleotides; proteins; acrylamide; nucleic acid; provided comprising; nucleic acids; oligonucleotide molecules; fluorescent dyes; dna molecules; dna molecule; fluorescent dye; chemically active; immobilizing biomolecules; /999/428/
Citation Formats
Mirzabekov, Andrei Darievich, Proudnikov, Dimitri Y, Timofeev, Edward N, Kochetkova, Svetlana V, Florentiev, Vladimir L, and Shick, Valentine V. Methods for immobilizing nucleic acids on a gel substrate. United States: N. p., 1999.
Web.
Mirzabekov, Andrei Darievich, Proudnikov, Dimitri Y, Timofeev, Edward N, Kochetkova, Svetlana V, Florentiev, Vladimir L, & Shick, Valentine V. Methods for immobilizing nucleic acids on a gel substrate. United States.
Mirzabekov, Andrei Darievich, Proudnikov, Dimitri Y, Timofeev, Edward N, Kochetkova, Svetlana V, Florentiev, Vladimir L, and Shick, Valentine V. Fri .
"Methods for immobilizing nucleic acids on a gel substrate". United States. https://www.osti.gov/servlets/purl/872654.
@article{osti_872654,
title = {Methods for immobilizing nucleic acids on a gel substrate},
author = {Mirzabekov, Andrei Darievich and Proudnikov, Dimitri Y and Timofeev, Edward N and Kochetkova, Svetlana V and Florentiev, Vladimir L and Shick, Valentine V},
abstractNote = {A method for labeling oligonucleotide molecules, and for immobilizing oligonucleotide and DNA molecules is provided comprising modifying the molecules to create a chemically active group, and contacting activated fluorescent dyes to the region. A method for preparing an immobilization substrate is also provided comprising modifying a gel to contain desired functional groups which covalently interact with certain moieties of the oligonucleotide molecules. A method for immobilizing biomolecules and other molecules within a gel by copolymerization of allyl-substituted oligonucleotides, DNA and proteins with acrylamide is also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
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Works referenced in this record:
Regioselective immobilization of short oligonucleotides to acrylic copolymer gels
journal, August 1996
- Timofeev, E.
- Nucleic Acids Research, Vol. 24, Issue 16
Chemical Methods of DNA and RNA Fluorescent Labeling
journal, November 1996
- Proudnikov, D.; Mirzabekov, A.
- Nucleic Acids Research, Vol. 24, Issue 22