Activated recombinant adenovirus proteinases
Abstract
This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying said peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described.
- Inventors:
-
- Stony Brook, NY
- Shoreham, NY
- Issue Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- OSTI Identifier:
- 872441
- Patent Number(s):
- 5935840
- Application Number:
- 08/435,998
- Assignee:
- Associated Universities, Inc. (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- activated; recombinant; adenovirus; proteinases; application; describes; methods; expression; constructs; producing; activatable; purified; purification; described; obtaining; included; isolated; peptide; cofactors; proteinase; activity; purifying; identifying; antibodies; immunoreactive; immunospecific; preparing; related; materials; related method; recombinant adenovirus; application describes; adenovirus proteinases; related methods; antibodies immunoreactive; isolated peptide; expression constructs; activatable recombinant; purified activatable; describes methods; producing activatable; activated recombinant; adenovirus proteinase; /435/999/
Citation Formats
Anderson, Carl W, and Mangel, Walter F. Activated recombinant adenovirus proteinases. United States: N. p., 1999.
Web.
Anderson, Carl W, & Mangel, Walter F. Activated recombinant adenovirus proteinases. United States.
Anderson, Carl W, and Mangel, Walter F. Fri .
"Activated recombinant adenovirus proteinases". United States. https://www.osti.gov/servlets/purl/872441.
@article{osti_872441,
title = {Activated recombinant adenovirus proteinases},
author = {Anderson, Carl W and Mangel, Walter F},
abstractNote = {This application describes methods and expression constructs for producing activatable recombinant adenovirus proteinases. Purified activatable recombinant adenovirus proteinases and methods of purification are described. Activated adenovirus proteinases and methods for obtaining activated adenovirus proteinases are further included. Isolated peptide cofactors of adenovirus proteinase activity, methods of purifying and identifying said peptide cofactors are also described. Antibodies immunoreactive with adenovirus proteinases, immunospecific antibodies, and methods for preparing them are also described. Other related methods and materials are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1999},
month = {Fri Jan 01 00:00:00 EST 1999}
}
Works referenced in this record:
Sequence of the human adenovirus type 3 protease
journal, January 1988
- Houde, Alain; Weber, Joseph M.
- Nucleic Acids Research, Vol. 16, Issue 23
Characterization of the adenovirus 2 virion protein, Mu
journal, October 1989
- Anderson, Carl W.; Young, Marjorie E.; Flint, S. J.
- Virology, Vol. 172, Issue 2
Nucleotide and deduced amino acid sequence of the bovine adenovirus type 7 proteinase
journal, January 1990
- Cai, Faxing; Tang, Denis; Hu, Shiu-Lok
- Nucleic Acids Research, Vol. 18, Issue 18
Expression and Purification of the Adenovirus Proteinase Polypeptide and of a Synthetic Proteinase Substrate
journal, February 1993
- Anderson, C. W.
- Protein Expression and Purification, Vol. 4, Issue 1
The primary structure of human adenovirus type 12 protease
journal, January 1988
- Houde, Alain; Weber, Joseph M.
- Nucleic Acids Research, Vol. 16, Issue 14
Adenovirus proteinases: comparison of amino acid sequences and expression of the cloned cDNA in Escherichia coli
journal, April 1990
- Houde, Alain; Weber, Joseph M.
- Gene, Vol. 88, Issue 2
Human Adenovirus Serotype 12 Virion Precursors pMu and pVI Are Cleaved at Amino-Terminal and Carboxy-Terminal Sites That Conform to the Adenovirus 2 Endoproteinase Cleavage Consensus Sequence
journal, March 1993
- Freimuth, Paul; Anderson, Carl W.
- Virology, Vol. 193, Issue 1
Adenovirus type 2 endopeptidase: an unusual phosphoprotein enzyme matured by autocatalysis.
journal, February 1987
- Chatterjee, P. K.; Flint, S. J.
- Proceedings of the National Academy of Sciences, Vol. 84, Issue 3
In vitro cleavage specificity of the adenovirus type 2 proteinase
journal, March 1983
- Tremblay, Michel L.; Déry, Claude V.; Talbot, Brian G.
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 743, Issue 2
Gly-Gly-X, a Novel Consensus Sequence for the Proteolytic Processing of Viral and Cellular Proteins
journal, June 1989
- López-Otín, C.; Simón-Mateo, C.; Martínez, L.
- Journal of Biological Chemistry, Vol. 264, Issue 16
Nucleotide and deduced amino acid sequence of the bovine adenovirus type 3 proteinase
journal, January 1990
- Cai, Faxing; Bourbonnière, Martin; Tang, Denis
- Nucleic Acids Research, Vol. 18, Issue 18
Adenovirus type 2 precursor proteins are cleaved by proteinases of other adenoviruses
journal, November 1990
- Houde, Alain; Weber, Joseph M.
- Virology, Vol. 179, Issue 1
The adenovirus protease is activated by a virus-coded disulphide-linked peptide
journal, January 1993
- Webster, Ailsa; Hay, Ronald T.; Kemp, Graham
- Cell, Vol. 72, Issue 1
Sequence of the protease of human subgroup E adenovirus type 4
journal, January 1987
- Houde, A.; Weber, J. M.
- Gene, Vol. 54, Issue 1
The active adenovirus protease is the intact L3 23K protein
journal, July 1993
- Webster, A.; Kemp, G.
- Journal of General Virology, Vol. 74, Issue 7
Characterization of the Adenovirus Proteinase: Substrate Specificity
journal, December 1989
- Webster, A.; Russell, S.; Talbot, P.
- Journal of General Virology, Vol. 70, Issue 12
Genetic identification of a endoproteinase endoded by the adenovirus genome
journal, June 1983
- Yeh-Kai, Liang; Akusjärvi, Göran; Aleström, Peter
- Journal of Molecular Biology, Vol. 167, Issue 1