Method for construction of normalized cDNA libraries
Abstract
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.
- Inventors:
-
- New York, NY
- Englewood, NJ
- Issue Date:
- Research Org.:
- Columbia Univ., New York, NY (United States)
- OSTI Identifier:
- 871950
- Patent Number(s):
- 5830662
- Assignee:
- Trustees of Columbia University in City of New York (New York, NY)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-91ER61233
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; construction; normalized; cdna; libraries; provides; normalize; directional; library; constructed; vector; allows; propagation; single-stranded; circle; form; comprising; propagating; circles; generating; fragments; complementary; noncoding; sequence; produce; partial; duplexes; purifying; melting; reassociating; purified; appropriate; cot; unassociated; generated; above-described; cdna libraries; produce partial; directional cdna; single-stranded circles; cdna library; partial duplexes; normalized cdna; purified partial; single-stranded circle; unassociated single-stranded; appropriate cot; noncoding sequence; circle form; allows propagation; coding sequence; library constructed; form comprising; generating fragments; fragments complementary; /435/999/
Citation Formats
Soares, Marcelo B, and Efstratiadis, Argiris. Method for construction of normalized cDNA libraries. United States: N. p., 1998.
Web.
Soares, Marcelo B, & Efstratiadis, Argiris. Method for construction of normalized cDNA libraries. United States.
Soares, Marcelo B, and Efstratiadis, Argiris. Thu .
"Method for construction of normalized cDNA libraries". United States. https://www.osti.gov/servlets/purl/871950.
@article{osti_871950,
title = {Method for construction of normalized cDNA libraries},
author = {Soares, Marcelo B and Efstratiadis, Argiris},
abstractNote = {This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to appropriate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. This invention also provides normalized cDNA libraries generated by the above-described method and uses of the generated libraries.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}
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Construction of a normalized cDNA library by introduction of a semi-solid mRNA-cDNA hybridization system
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