Hybrid slab-microchannel gel electrophoresis system
Abstract
A hybrid slab-microchannel gel electrophoresis system. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate.
- Inventors:
-
- Livermore, CA
- San Ramon, CA
- Issue Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- OSTI Identifier:
- 871521
- Patent Number(s):
- 5746901
- Assignee:
- Regents Of University Of California (Oakland, CA)
- Patent Classifications (CPCs):
-
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- hybrid; slab-microchannel; electrophoresis; permits; fabrication; isolated; microchannels; biomolecule; separations; imposing; constraint; totally; sealed; reusable; ultimately; simpler; costly; manufacture; closed; channel; plate; incorporates; microslab; portion; separation; medium; substantially; reducing; possibility; non-uniform; field; distribution; breakdown; due; uncontrollable; leakage; sieving; matrix; built; plastic; spacer; materials; uniformly; couple; top; bottom; microchannel; channel plate; microchannel plate; top plate; substantially reducing; spacer material; uniform field; hybrid slab-microchannel; field distribution; /204/
Citation Formats
Balch, Joseph W, Carrano, Anthony V, Davidson, James C, and Koo, Jackson C. Hybrid slab-microchannel gel electrophoresis system. United States: N. p., 1998.
Web.
Balch, Joseph W, Carrano, Anthony V, Davidson, James C, & Koo, Jackson C. Hybrid slab-microchannel gel electrophoresis system. United States.
Balch, Joseph W, Carrano, Anthony V, Davidson, James C, and Koo, Jackson C. Thu .
"Hybrid slab-microchannel gel electrophoresis system". United States. https://www.osti.gov/servlets/purl/871521.
@article{osti_871521,
title = {Hybrid slab-microchannel gel electrophoresis system},
author = {Balch, Joseph W and Carrano, Anthony V and Davidson, James C and Koo, Jackson C},
abstractNote = {A hybrid slab-microchannel gel electrophoresis system. The hybrid system permits the fabrication of isolated microchannels for biomolecule separations without imposing the constraint of a totally sealed system. The hybrid system is reusable and ultimately much simpler and less costly to manufacture than a closed channel plate system. The hybrid system incorporates a microslab portion of the separation medium above the microchannels, thus at least substantially reducing the possibility of non-uniform field distribution and breakdown due to uncontrollable leakage. A microslab of the sieving matrix is built into the system by using plastic spacer materials and is used to uniformly couple the top plate with the bottom microchannel plate.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Thu Jan 01 00:00:00 EST 1998},
month = {Thu Jan 01 00:00:00 EST 1998}
}