Method and apparatus for detection of fluorescently labeled materials
Abstract
Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.
- Inventors:
-
- Mt. View, CA
- Los Altos, CA
- Issue Date:
- Research Org.:
- Affymax Research Institute
- OSTI Identifier:
- 870962
- Patent Number(s):
- 5631734
- Assignee:
- Affymetrix, Inc. (Santa Clara, CA)
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- DOE Contract Number:
- FG03-92ER81275
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; apparatus; detection; fluorescently; labeled; materials; marked; targets; bind; substrate; 230; synthesized; polymer; sequences; locations; detected; exposing; selected; regions; light; source; 100; detecting; photons; fluoresced; therefrom; repeating; steps; exposure; completely; examined; resulting; data; determine; binding; affinity; specific; light source; selected regions; resulting data; fluorescently labeled; selected region; specific polymer; /356/250/
Citation Formats
Stern, David, and Fiekowsky, Peter. Method and apparatus for detection of fluorescently labeled materials. United States: N. p., 1997.
Web.
Stern, David, & Fiekowsky, Peter. Method and apparatus for detection of fluorescently labeled materials. United States.
Stern, David, and Fiekowsky, Peter. Wed .
"Method and apparatus for detection of fluorescently labeled materials". United States. https://www.osti.gov/servlets/purl/870962.
@article{osti_870962,
title = {Method and apparatus for detection of fluorescently labeled materials},
author = {Stern, David and Fiekowsky, Peter},
abstractNote = {Fluorescently marked targets bind to a substrate 230 synthesized with polymer sequences at known locations. The targets are detected by exposing selected regions of the substrate 230 to light from a light source 100 and detecting the photons from the light fluoresced therefrom, and repeating the steps of exposure and detection until the substrate 230 is completely examined. The resulting data can be used to determine binding affinity of the targets to specific polymer sequences.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 1997},
month = {Wed Jan 01 00:00:00 EST 1997}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Features and Applications of the Laser Scanning Microscope
journal, November 1990
- Yamamoto, Kimiaki; Horikawa, Yoshiaki; Yamamoto, Mitsunori
- Journal of Modern Optics, Vol. 37, Issue 11
Confocal compact scanning optical microscope based on compact disc technology
journal, January 1991
- Benschop, Jos; van Rosmalen, Gerard
- Applied Optics, Vol. 30, Issue 10
Development of microspot multi-analyte ratiometric immunoassay using dual fluorescent-labelled antibodies
journal, January 1989
- Ekins, Roger; Chu, Frederick; Biggart, Elizabeth
- Analytica Chimica Acta, Vol. 227
An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy.
journal, July 1987
- White, J. G.; Amos, W. B.; Fordham, M.
- The Journal of Cell Biology, Vol. 105, Issue 1
Multianalyte microspot immunoassay--microanalytical "compact disk" of the future
journal, November 1991
- Ekins, R. P.; Chu, F. W.
- Clinical Chemistry, Vol. 37, Issue 11
Design and use of a computer controlled confocal microscope for biological applications: Confocal microscope for biological applications
journal, January 1985
- van der Voort, H. T. M.; Brakenhoff, G. J.; Valkenburg, J. A. C.
- Scanning, Vol. 7, Issue 2