Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus
Abstract
A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.
- Inventors:
-
- Littleton, CO
- Lakewood, CO
- Golden, CO
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 869971
- Patent Number(s):
- 5432075
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC36-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- molecular; weight; thermostable; beta; -d-glucosidase; acidothermus; cellulolyticus; purified; produced; atcc; 43068; enzyme; water; soluble; possesses; activity; pnp-; -d-glucopyranoside; degree; stability; heat; exhibits; optimal; temperature; 65; ph; range; inactivation; 80; 50; 5-54; kd; determineded; sds-page; ph range; cellulolyticus atcc; water soluble; molecular weight; acidothermus cellulolyticus; temperature activity; inactivation temperature; /435/
Citation Formats
Himmel, Michael E, Tucker, Melvin P, Adney, William S, and Nieves, Rafael A. Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus. United States: N. p., 1995.
Web.
Himmel, Michael E, Tucker, Melvin P, Adney, William S, & Nieves, Rafael A. Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus. United States.
Himmel, Michael E, Tucker, Melvin P, Adney, William S, and Nieves, Rafael A. Sun .
"Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus". United States. https://www.osti.gov/servlets/purl/869971.
@article{osti_869971,
title = {Low molecular weight thermostable .beta.-D-glucosidase from acidothermus cellulolyticus},
author = {Himmel, Michael E and Tucker, Melvin P and Adney, William S and Nieves, Rafael A},
abstractNote = {A purified low molecular weight .beta.-D-glucosidase is produced from Acidothermus cellulolyticus ATCC 43068. The enzyme is water soluble, possesses activity against pNP-.beta.-D-glucopyranoside, has a high of degree of stability toward heat, exhibits optimal temperature activity at about 65.degree. C. at a pH range of from about 2 to about 7, has an inactivation temperature of about 80.degree. C. at a pH range of from about 2 to about 7 and has a molecular weight of about 50.5-54.5 kD as determineded by SDS-PAGE.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1995},
month = {Sun Jan 01 00:00:00 EST 1995}
}
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