DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Thermostable purified endoglucanase II from Acidothermus cellulolyticus ATCC

Abstract

A purified low molecular weight endoglucanase II from Acidothermus cellulolyticus (ATCC 43068) is disclosed. The endoglucanase is water soluble, possesses both C.sub.1, and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 81.degree. C. at pH's from about 2 to about 9, and at a inactivation temperature of about 100.degree. C. at pH's from about 2 to about 9.

Inventors:
 [1];  [2];  [3];  [4]
  1. Golden, CO
  2. Denver, CO
  3. Lakewood, CO
  4. Littleton, CO
Issue Date:
Research Org.:
Midwest Research Institute, Kansas City, MO (United States)
OSTI Identifier:
869615
Patent Number(s):
5366884
Assignee:
Midwest Research Institute (Kansas City, MO)
Patent Classifications (CPCs):
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
AC02-83CH10093
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
thermostable; purified; endoglucanase; ii; acidothermus; cellulolyticus; atcc; molecular; weight; 43068; disclosed; water; soluble; possesses; types; enzyme; activity; degree; stability; heat; exhibits; optimum; temperature; 81; ph; inactivation; 100; cellulolyticus atcc; optimum temperature; enzyme activity; water soluble; molecular weight; acidothermus cellulolyticus; thermostable purified; temperature activity; purified endoglucanase; inactivation temperature; endoglucanase ii; purified endoglucanas; /435/

Citation Formats

Adney, William S, Thomas, Steven R, Nieves, Rafael A, and Himmel, Michael E. Thermostable purified endoglucanase II from Acidothermus cellulolyticus ATCC. United States: N. p., 1994. Web.
Adney, William S, Thomas, Steven R, Nieves, Rafael A, & Himmel, Michael E. Thermostable purified endoglucanase II from Acidothermus cellulolyticus ATCC. United States.
Adney, William S, Thomas, Steven R, Nieves, Rafael A, and Himmel, Michael E. Sat . "Thermostable purified endoglucanase II from Acidothermus cellulolyticus ATCC". United States. https://www.osti.gov/servlets/purl/869615.
@article{osti_869615,
title = {Thermostable purified endoglucanase II from Acidothermus cellulolyticus ATCC},
author = {Adney, William S and Thomas, Steven R and Nieves, Rafael A and Himmel, Michael E},
abstractNote = {A purified low molecular weight endoglucanase II from Acidothermus cellulolyticus (ATCC 43068) is disclosed. The endoglucanase is water soluble, possesses both C.sub.1, and C.sub.x types of enzyme activity, a high degree of stability toward heat, and exhibits optimum temperature activity at about 81.degree. C. at pH's from about 2 to about 9, and at a inactivation temperature of about 100.degree. C. at pH's from about 2 to about 9.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Sat Jan 01 00:00:00 EST 1994},
month = {Sat Jan 01 00:00:00 EST 1994}
}

Works referenced in this record:

Cellulolytic enzyme system of Acetivibrio cellulolyticus
journal, March 1981


The Production of Cellulases
book, June 1969


Mechanisms of Thermophily
journal, January 1978


Isolation and Characterization of Acidothermus cellulolyticus gen. nov., sp. nov., a New Genus of Thermophilic, Acidophilic, Cellulolytic Bacteria
journal, July 1986