Fiber optic-based regenerable biosensor
Abstract
A fiber optic-based regenerable biosensor. The biosensor is particularly suitable for use in microscale work in situ. In one embodiment, the biosensor comprises a reaction chamber disposed adjacent the distal end of a waveguide and adapted to receive therein a quantity of a sample containing an analyte. Leading into the chamber is a plurality of capillary conduits suitable for introducing into the chamber antibodies or other reagents suitable for selective interaction with a predetermined analyte. Following such interaction, the contents of the chamber may be subjected to an incident energy signal for developing fluorescence within the chamber that is detectable via the optical fiber and which is representative of the presence, i.e. concentration, of the selected analyte. Regeneration of the biosensor is accomplished by replacement of the reagents and/or the analyte, or a combination of these, at least in part via one or more of the capillary conduits. The capillary conduits extend from their respective terminal ends that are in fluid communication with the chamber, away from the chamber to respective location(s) remote from the chamber thereby permitting in situ location of the chamber and remote manipulation and/or analysis of the activity with the chamber.
- Inventors:
-
- Knoxville, TN
- Issue Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- OSTI Identifier:
- 868619
- Patent Number(s):
- 5176881
- Assignee:
- University of Tennessee Research Corporation (Knoxville, TN)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- fiber; optic-based; regenerable; biosensor; particularly; suitable; microscale; situ; embodiment; comprises; reaction; chamber; disposed; adjacent; distal; waveguide; adapted; receive; therein; quantity; sample; containing; analyte; leading; plurality; capillary; conduits; introducing; antibodies; reagents; selective; interaction; predetermined; following; contents; subjected; incident; energy; signal; developing; fluorescence; detectable; via; optical; representative; presence; concentration; selected; regeneration; accomplished; replacement; combination; extend; respective; terminal; fluid; communication; location; remote; permitting; manipulation; analysis; activity; chamber disposed; incident energy; sample containing; disposed adjacent; particularly suitable; reaction chamber; fiber optic; optical fiber; fluid communication; sensor comprises; remote manipulation; biosensor comprises; regenerable biosensor; reagents suitable; optic-based regenerable; fiber optic-based; /422/356/600/
Citation Formats
Sepaniak, Michael J, and Vo-Dinh, Tuan. Fiber optic-based regenerable biosensor. United States: N. p., 1993.
Web.
Sepaniak, Michael J, & Vo-Dinh, Tuan. Fiber optic-based regenerable biosensor. United States.
Sepaniak, Michael J, and Vo-Dinh, Tuan. Fri .
"Fiber optic-based regenerable biosensor". United States. https://www.osti.gov/servlets/purl/868619.
@article{osti_868619,
title = {Fiber optic-based regenerable biosensor},
author = {Sepaniak, Michael J and Vo-Dinh, Tuan},
abstractNote = {A fiber optic-based regenerable biosensor. The biosensor is particularly suitable for use in microscale work in situ. In one embodiment, the biosensor comprises a reaction chamber disposed adjacent the distal end of a waveguide and adapted to receive therein a quantity of a sample containing an analyte. Leading into the chamber is a plurality of capillary conduits suitable for introducing into the chamber antibodies or other reagents suitable for selective interaction with a predetermined analyte. Following such interaction, the contents of the chamber may be subjected to an incident energy signal for developing fluorescence within the chamber that is detectable via the optical fiber and which is representative of the presence, i.e. concentration, of the selected analyte. Regeneration of the biosensor is accomplished by replacement of the reagents and/or the analyte, or a combination of these, at least in part via one or more of the capillary conduits. The capillary conduits extend from their respective terminal ends that are in fluid communication with the chamber, away from the chamber to respective location(s) remote from the chamber thereby permitting in situ location of the chamber and remote manipulation and/or analysis of the activity with the chamber.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Fri Jan 01 00:00:00 EST 1993},
month = {Fri Jan 01 00:00:00 EST 1993}
}
Works referenced in this record:
Development of antibody-based fiber-optic sensors for detection of a benzo[a]pyrene metabolite
journal, September 1988
- Tromberg, Bruce J.; Sepaniak, Michael J.; Alarie, Jean Pierre.
- Analytical Chemistry, Vol. 60, Issue 18
The clinical use of laser-excited fluorometry.
journal, May 1985
- Sepaniak, M. J.
- Clinical Chemistry, Vol. 31, Issue 5