Method of saccharifying cellulose
Abstract
A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.
- Inventors:
-
- Brookline, MA
- Wellesley, MA
- Decatur, IL
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- OSTI Identifier:
- 865590
- Patent Number(s):
- 4540664
- Application Number:
- 06/494,482
- Assignee:
- United States of America as represented by United States (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- EG-77-C-02-4198; XR-9-8109-1
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- method; saccharifying; cellulose; incubation; cellulase; clostridium; thermocellum; broth; containing; efficacious; amount; reducing; agent; parameters; advantageously; controlled; stimulate; saccharification; concentration; alkaline; earth; salts; ph; temperature; duration; native; crystalline; found; cotton; completely; saccharified; crystalline cellulose; alkaline earth; reducing agent; earth salts; saccharifying cellulose; clostridium thermocellum; /435/
Citation Formats
Johnson, Eric A, Demain, Arnold L, and Madia, Ashwin. Method of saccharifying cellulose. United States: N. p., 1985.
Web.
Johnson, Eric A, Demain, Arnold L, & Madia, Ashwin. Method of saccharifying cellulose. United States.
Johnson, Eric A, Demain, Arnold L, and Madia, Ashwin. Tue .
"Method of saccharifying cellulose". United States. https://www.osti.gov/servlets/purl/865590.
@article{osti_865590,
title = {Method of saccharifying cellulose},
author = {Johnson, Eric A and Demain, Arnold L and Madia, Ashwin},
abstractNote = {A method of saccharifying cellulose by incubation with the cellulase of Clostridium thermocellum in a broth containing an efficacious amount of a reducing agent. Other incubation parameters which may be advantageously controlled to stimulate saccharification include the concentration of alkaline earth salts, pH, temperature, and duration. By the method of the invention, even native crystalline cellulose such as that found in cotton may be completely saccharified.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Sep 10 00:00:00 EDT 1985},
month = {Tue Sep 10 00:00:00 EDT 1985}
}
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