Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains
Abstract
This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1805627
- Patent Number(s):
- 10947548
- Application Number:
- 16/393,149
- Assignee:
- Battelle Memorial Institute (Richland, WA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC05-76RL01830
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 04/24/2019
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Deng, Shuang, Dai, Ziyu, and Magnuson, Jon K. Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains. United States: N. p., 2021.
Web.
Deng, Shuang, Dai, Ziyu, & Magnuson, Jon K. Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains. United States.
Deng, Shuang, Dai, Ziyu, and Magnuson, Jon K. Tue .
"Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains". United States. https://www.osti.gov/servlets/purl/1805627.
@article{osti_1805627,
title = {Production of organic acids from Aspergillus cis-aconitic acid decarboxylase (cadA) deletion strains},
author = {Deng, Shuang and Dai, Ziyu and Magnuson, Jon K.},
abstractNote = {This application provides recombinant Aspergillus fungi having an endogenous cis-aconitic acid decarboxylase (cadA) gene genetically inactivated, which allows aconitic acid production by the recombinant fungi. Such recombinant fungi can further include an exogenous nucleic acid molecule encoding aspartate decarboxylase (panD), an exogenous nucleic acid molecule encoding β-alanine-pyruvate aminotransferase (BAPAT), and an exogenous nucleic acid molecule encoding 3-hydroxypropionate dehydrogenase (HPDH). Kits including these fungi, and methods of using these fungi to produce aconitic acid and 3-hydroxypropionic acid (3-HP) are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 16 00:00:00 EDT 2021},
month = {Tue Mar 16 00:00:00 EDT 2021}
}
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Works referenced in this record:
Fermentation process and microorganism for producing aconitic acid
patent, April 1988
- Holdom, Kelvin S.; Winskill, Norman
- US Patent Document 4,740,464
Methods and Organisms for Growth-Coupled Production of 3-Hydroxypropionic Acid
patent-application, August 2008
- Burgard, Anthony P.; Van Dien, Stephen J.
- 12/018129; 20080199926
Genetically Engineered Yeast
patent-application, September 2015
- Borodina, Irina; Kildegaard, Kanchana Rueksomtawin; Forster, Jochen
- 14/435111; 20150267228