Title: RNA-guided transcriptional regulation

Abstract

Methods of modulating expression of a target nucleic acid in a cell are provided including introducing into the cell a first foreign nucleic acid encoding one or more RNAs complementary to DNA, wherein the DNA includes the target nucleic acid, introducing into the cell a second foreign nucleic acid encoding a nuclease-null Cas9 protein that binds to the DNA and is guided by the one or more RNAs, introducing into the cell a third foreign nucleic acid encoding a transcriptional regulator protein or domain, wherein the one or more RNAs, the nuclease-null Cas9 protein, and the transcriptional regulator protein or domain are expressed, wherein the one or more RNAs, the nuclease-null Cas9 protein and the transcriptional regulator protein or domain co-localize to the DNA and wherein the transcriptional regulator protein or domain regulates expression of the target nucleic acid.

Inventors:

Church, George M.; Mali, Prashant G.; Esvelt, Kevin M.

Issue Date:

Tue Sep 08 00:00:00 EDT 2020

Research Org.:

Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1735230

Patent Number(s):

10767194

Application Number:

16/441,209

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/102 - {Mutagenizing nucleic acids}
C12N15/11 - DNA or RNA fragments
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/635 - {Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline}
C12N15/907 - {in mammalian cells}
C12N2310/20 - involving clustered regularly interspaced short palindromic repeats [CRISPRs]
C12N2310/3513 - Protein
C12N9/22 - Ribonucleases {RNAses, DNAses

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y301/00 - Hydrolases acting on ester bonds

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DOE Contract Number:  

FG02-02ER63445

Resource Type:

Patent

Resource Relation:

Patent File Date: 06/14/2019

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES